17-Beta-Hydroxysteroid Dehydrogenase Type 3, Polyclonal Antibody

SDR12C2; EDH17B3; Short Chain Dehydrogenase/Reductase Family 12C,Member 2; Testosterone 17-Beta-Dehydrogenase 3; Testicular 17-beta-hydroxysteroid dehydrogenase

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against HSD17b3. It has been selected for its ability to recognize HSD17b3 in immunohistochemical staining and western blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of anti-HSD17b3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000

Western Blot: Sample: Recombinant HSD17b3, Human.

DAB staining on fromalin fixed paraffin- embedded testis tissue)

29,109 Da

hydroxysteroid 17-beta dehydrogenase 3

testosterone 17-beta-dehydrogenase 3

Testosterone 17-beta-dehydrogenase 3

EDH17B3; SDR12C2; 17-beta-HSD 3  [Similar Products]

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]

HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: EC 1.1.1.64; Lipid Metabolism - androgen and estrogen; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q22.32

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: testosterone 17-beta-dehydrogenase (NADP+) activity; testosterone dehydrogenase (NAD+) activity

Biological Process: androgen biosynthetic process; male genitalia development

Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency

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