Product Name
SHANK1/SHANK3, Monoclonal Antibody
Full Product Name
SHANK1/SHANK3 Antibody, Clone S367-51: Dylight 488
Product Synonym Names
Mouse Anti-Rat SHANK1/SHANK3 Monoclonal IgG2a; SHANK1/SHANK3 Antibody: Dylight 488; Shank postsynaptic density protein Antibody; SH3 and multiple ankyrin repeat domains 3 Antibody; Proline rich synapse associated protein 2 Antibody; Proline-rich synapse-associated protein 2 Antibody; ProSAP2 Antibody; PSAP2 Antibody; Antibody; SH3 and multiple ankyrin repeat domains protein 3 Antibody; SH3/ankyrin domain gene 3,Shank3b Antibody; SPANK 2 Antibody; SPANK2 Antibody; KAP/SAPAP interacting protein Antibody; OTTHUMP00000174437 Antibody; SH3 and multiple ankyrin repeat domains 1 Antibody; SH3 and multiple ankyrin repeat domains protein 1 Antibody; SH3/ankyrin domain gene 1 Antibody; SHANK 1 Antibody; Shank1 Antibody; Shank1a Antibody; Somatostatin receptor interacting protein Antibody; Somatostatin receptor-interacting protein Antibody; SPANK 1 Antibody; SPANK1 Antibody; SSTR interacting protein Antibody; SSTR-interacting protein Antibody; SSTRIP Antibody; Synamon Antibody; AI841104 Antibody; DEL22q13.3 Antibody; KIAA1650 Antibody
Product Gene Name
anti-SHANK1/SHANK3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9JLU4
Species Reactivity
Human, Mouse, Rat
Specificity
Detects ~190 kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.
Purity/Purification
Protein G Purified
Form/Format
PBS pH 7.4, 50% Glycerol, 0.1% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3. Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.
Cellular Localization
Cytoplasm; Cell Junction
Certificate of Analysis
1 ug/ml was sufficient for detection of SHANK1/SHANK3 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, Förster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-SHANK1/SHANK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SHANK1/SHANK3 antibody
SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of ***** and developing brain to ensure normal development.
Product Categories/Family for anti-SHANK1/SHANK3 antibody
Neuroscience; Cell Markers; Neuron Markers; Postsynaptic Markers; Cell Signaling; Scaffold Proteins
Applications Tested/Suitable for anti-SHANK1/SHANK3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-SHANK1/SHANK3 antibody
WB: 1:1000
ICC/IF: 1:100
Immunocytochemistry (ICC) of anti-SHANK1/SHANK3 antibody
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK1/SHANK3 Antibody. (D) Composite.
Western Blot (WB) of anti-SHANK1/SHANK3 antibody
Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.
NCBI/Uniprot data below describe general gene information for SHANK1/SHANK3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_067708.1
[Other Products]
NCBI GenBank Nucleotide #
NP_067708.1
[Other Products]
UniProt Primary Accession #
Q9JLU4
[Other Products]
UniProt Related Accession #
Q9JLU4[Other Products]
NCBI Official Full Name
SH3 and multiple ankyrin repeat domains protein 3
NCBI Official Synonym Full Names
SH3 and multiple ankyrin repeat domains 3
NCBI Official Symbol
Shank3 [Similar Products]
NCBI Official Synonym Symbols
Prosap2; Spank-2
[Similar Products]
NCBI Protein Information
SH3 and multiple ankyrin repeat domains protein 3
UniProt Protein Name
SH3 and multiple ankyrin repeat domains protein 3
UniProt Synonym Protein Names
Proline-rich synapse-associated protein 2; ProSAP2; SPANK-2
UniProt Gene Name
Shank3 [Similar Products]
UniProt Synonym Gene Names
Prosap2; Shank3; ProSAP2 [Similar Products]
NCBI Summary for SHANK1/SHANK3
may be involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]
UniProt Comments for SHANK1/SHANK3
Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.
Research Articles on SHANK1/SHANK3
1. Loss of scaffold proteins via knockdown of Shank1 or Shank2, but not Shank3, led to a reduction of the number but not the unitary response of AMPAR-containing synapses. Only when both Shank1 and Shank2 were knocked down, were both the number and the unitary response of active synapses reduced. This reduction was accompanied by a decrease in NMDAR-mediated synaptic response.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
