b2-Microglobulin, Polyclonal Antibody

b2-Microglobulin (B2M)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 6; NC_000006.11 (29794756..29798899). Location: 6p21.3

Polyclonal

Host: Rabbit
Source: Human

Recognizes human B2M.

Serum

Serum
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-HLA-G antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600 (human 119 aa, chromosome 15). B2M associates with heavy hcain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene.

Abs to Serum Proteins

ELISA, Western Blot

Suitable for use in Western Blot and ELISA. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 using Chemiluminescence technique. B2M is ~12kD. See M3890-55B for positive control.
ELISA: 1:100,000; using 50-100ng control antigen/well.
Optimal dilutions to be determined by the researcher.

31,549 Da[Similar Products]

major histocompatibility complex, class I, G

HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; OTTHUMP00000029218; OTTHUMP00000029220; OTTHUMP00000033527; OTTHUMP00000159507; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G

HLA-G protein

O02923_HUMAN

• (1).  Gussow D et al (1987) J. Immunol. 139, 3132-3138; Suggs SV et al (1981) PNAS 78, 6613-6617; Cunningham BA et al (1973) Biochemistry 12, 4811-4822; Bjorkman PJ et al (1987) nature 329, 506-512; deSouza M et al (1994) Immunol. Lett. 39, 105-111

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.