17-Alpha Hydroxylase, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 10; NC_000010.10 (104590288..104597290, complement). Location: 10q24.3

This assay has high sensitivity and excellent specificity for detection of S17AH. No significant cross-reactivity or interference between S17AH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S17AH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of S17AH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS737160 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 17-Alpha Hydroxylase (S17AH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing S17AH. The ELISA analytical biochemical technique of the MBS737160 kit is based on S17AH antibody-S17AH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S17AH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S17AH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: S17AH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-S17AH antibody and an S17AH-HRP conjugate. The assay sample and buffer are incubated together with S17AH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the S17AH concentration since S17AH from samples and S17AH-HRP conjugate compete for the anti-S17AH antibody . Since the number of sites is limited, as more sites are occupied by S17AH from the sample, fewer sites are left to bind S17AH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S17AH concentration in each sample is interpolated from this standard curve.

Metabolism

57,371 Da

cytochrome P450, family 17, subfamily A, polypeptide 1

steroid 17-alpha-hydroxylase/17,20 lyase; CYPXVII; cytochrome P450c17; cytochrome P450-C17; cytochrome P450 17A1; cytochrome p450 XVIIA1; steroid 17-alpha-monooxygenase; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia

Steroid 17-alpha-hydroxylase/17,20 lyase

CYP17; S17AH; Cytochrome P450c17  [Similar Products]

CP17A_HUMAN

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]

CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short ***** stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; EC 4.1.2.30; EC 1.14.99.9; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 10q24.3

Cellular Component: endoplasmic reticulum membrane; cell soma; mitochondrion; axon; endoplasmic reticulum

Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; steroid 17-alpha-monooxygenase activity; iron ion binding; heme binding; oxygen binding

Biological Process: steroid metabolic process; response to cAMP; dibenzo-p-dioxin metabolic process; progesterone metabolic process; androgen biosynthetic process; response to insecticide; Leydig cell differentiation; biphenyl metabolic process; response to methylmercury; hormone biosynthetic process; response to nutrient levels; response to drug; response to retinoic acid; adrenal gland development; hippocampus development; phthalate metabolic process; response to herbicide; ovulation; response to acetate; response to steroid hormone stimulus; response to cytokine stimulus; xenobiotic metabolic process; response to ionizing radiation; glucocorticoid biosynthetic process; sterol metabolic process; sex differentiation; steroid biosynthetic process; phenol metabolic process

Disease: Adrenal Hyperplasia, Congenital, Due To 17-alpha-hydroxylase Deficiency

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