Product Synonym Names
Mismatch repair endonuclease PMS2; DNA mismatch repair protein PMS2; PMS1 protein homolog 2
Product Gene Name
anti-PMS2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P54278
Purity/Purification
Tissue culture supernatant
Storage Buffer
1*TBS (pH7.4), 0.5%BSA, 40% Glycerol.
Preservative
0.05% Sodium Azide.
Preparation and Storage
Aliquot and store at-20 degree C. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-PMS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PMS2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
NCBI/Uniprot data below describe general gene information for PMS2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000526.2
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NCBI GenBank Nucleotide #
NM_000535.6
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UniProt Primary Accession #
P54278
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UniProt Secondary Accession #
Q52LH6; Q5FBW9; Q5FBX1; Q5FBX2; Q75MR2; B2R610[Other Products]
UniProt Related Accession #
P54278[Other Products]
Molecular Weight
20,073 Da
NCBI Official Full Name
mismatch repair endonuclease PMS2 isoform a
NCBI Official Synonym Full Names
PMS1 homolog 2, mismatch repair system component
NCBI Official Symbol
PMS2 [Similar Products]
NCBI Official Synonym Symbols
MLH4; PMSL2; HNPCC4; PMS2CL
[Similar Products]
NCBI Protein Information
mismatch repair endonuclease PMS2
UniProt Protein Name
Mismatch repair endonuclease PMS2
UniProt Synonym Protein Names
DNA mismatch repair protein PMS2; PMS1 protein homolog 2
Protein Family
Mismatch repair endonuclease
UniProt Gene Name
PMS2 [Similar Products]
NCBI Summary for PMS2
The protein encoded by this gene is a key component of the mismatch repair system that functions to correct DNA mismatches and small insertions and deletions that can occur during DNA replication and homologous recombination. This protein forms heterodimers with the gene product of the mutL homolog 1 (MLH1) gene to form the MutL-alpha heterodimer. The MutL-alpha heterodimer possesses an endonucleolytic activity that is activated following recognition of mismatches and insertion/deletion loops by the MutS-alpha and MutS-beta heterodimers, and is necessary for removal of the mismatched DNA. There is a DQHA(X)2E(X)4E motif found at the C-terminus of the protein encoded by this gene that forms part of the active site of the nuclease. Mutations in this gene have been associated with hereditary nonpolyposis colorectal cancer (HNPCC; also known as Lynch syndrome) and Turcot syndrome. [provided by RefSeq, Apr 2016]
UniProt Comments for PMS2
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
Research Articles on PMS2
1. PCNA and MutLalpha interact specifically but weakly in solution to form a complex of approximately 1:1 stoichiometry that depends on PCNA interaction with the C-terminal endonuclease domain of the MutLalpha PMS2 subunit.
Precautions
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