Product Name
CD88 (C5AR1), Monoclonal Antibody
Full Product Name
CD88, phosphorylated (Ser334) (CD88 Antigen, Anaphylatoxin Receptor, C5a Anaphylatoxin Chemotactic Receptor, C5aR, C5a-R, C5a Ligand, C5R1)
Product Synonym Names
Anti -CD88, phosphorylated (Ser334) (CD88 Antigen, Anaphylatoxin Receptor, C5a Anaphylatoxin Chemotactic Receptor, C5aR, C5a-R, C5a Ligand, C5R1)
Product Gene Name
anti-C5AR1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 19; NC_000019.9 (47813104..47825327). Location: 19q13.3-q13.4
3D Structure
ModBase 3D Structure for P21730
Specificity
Recognizes human CD88 when phosphorylated at Ser334.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Immunogen
Synthetic phosphopeptide corresponding to 18 C terminal aa of human CD88, phosphorylated at Serine 332, 334 and 338.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-C5AR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-C5AR1 antibody
Antibodies; Abs to CD Markers
Applications Tested/Suitable for anti-C5AR1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-C5AR1 antibody
Suitable for use in ELISA, Immunofluorescence, and Western Blot.
Dilution: ELISA: 1:500-1:1,000
Immunofluorescence: 1:10-1:1,000
NCBI/Uniprot data below describe general gene information for C5AR1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001727.1
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NCBI GenBank Nucleotide #
NM_001736.3
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UniProt Primary Accession #
P21730
[Other Products]
UniProt Related Accession #
P21730[Other Products]
Molecular Weight
39,336 Da[Similar Products]
NCBI Official Full Name
C5a anaphylatoxin chemotactic receptor 1
NCBI Official Synonym Full Names
complement component 5a receptor 1
NCBI Official Symbol
C5AR1 [Similar Products]
NCBI Official Synonym Symbols
C5A; C5AR; C5R1; CD88
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NCBI Protein Information
C5a anaphylatoxin chemotactic receptor 1; C5a-R; C5a ligand; C5a anaphylatoxin receptor; complement component 5 receptor 1
UniProt Protein Name
C5a anaphylatoxin chemotactic receptor 1
UniProt Synonym Protein Names
C5a anaphylatoxin chemotactic receptor
Protein Family
C5a anaphylatoxin chemotactic receptor
UniProt Gene Name
C5AR1 [Similar Products]
UniProt Synonym Gene Names
C5AR; C5R1; C5a-R; C5aR [Similar Products]
UniProt Entry Name
C5AR1_HUMAN
UniProt Comments for C5AR1
C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.
Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: cell surface; basolateral plasma membrane; integral to plasma membrane; apical part of cell; cytoplasmic membrane-bound vesicle; plasma membrane
Molecular Function: complement component C5a binding; complement component C5a receptor activity; C5a anaphylatoxin receptor activity
Biological Process: neutrophil chemotaxis; organ regeneration; sensory perception of chemical stimulus; apoptosis; activation of MAPK activity; response to lipopolysaccharide; signal transduction; cell proliferation in hindbrain; chemotaxis; mRNA transcription from RNA polymerase II promoter; defense response to Gram-positive bacterium; elevation of cytosolic calcium ion concentration; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation
Research Articles on C5AR1
1. C5aR-C5L2 homodimerization versus heterodimerization contribute to differential responses obtained following ASP vs C5a stimulation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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