Full Product Name
Mouse Anti Human CD88: RPE
Product Synonym Names
CD88 antibody (P12/1); Mouse anti Human CD88
Product Gene Name
anti-CD88 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P21730
Species Reactivity
Human, Rhesus Monkey
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
1 mg/ml (lot specific)
Immunogen
C5aR-peptide: Met1-Asn31
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag8 myeloma cell line.
Preservative Stabilizers
0.09% Sodium Azide
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Other Notes
Small volumes of anti-CD88 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CD88 antibody
Mouse anti Human CD88 antibody, clone P12/1 recognizes the C5a receptor (C5aR) also known as CD88 or C5a anaphylatoxin chemotactic receptor 1. CD88 is predominantly expressed on cells of the myeloid lineage.
Mouse anti Human CD88 antibody, clone P12/1 does not inhibit the binding of C5a to its receptor.
Applications Tested/Suitable for anti-CD88 antibody
Flow Cytometry (FC/FACS)
Testing Data of anti-CD88 antibody
Figure A. Alexa Fluor647 conjugated mouse anti human CD16 and RPE conjugated mouse IgG2a isotype control . Figure B. Alexa Fluor647 conjugated mouse anti human CD16 and RPE conjugated mouse anti human CD88 . All experiments performed on red cell lysed human blood gated on granulocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.
Testing Data of anti-CD88 antibody
FFPE human spleen stained with Mouse Anti-Human CD88 , biotinylated Anti-Mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.
NCBI/Uniprot data below describe general gene information for CD88. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001727.1
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NCBI GenBank Nucleotide #
NM_001736.3
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UniProt Primary Accession #
P21730
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UniProt Related Accession #
P21730[Other Products]
NCBI Official Full Name
C5a anaphylatoxin chemotactic receptor 1
NCBI Official Synonym Full Names
complement C5a receptor 1
NCBI Official Symbol
C5AR1 [Similar Products]
NCBI Official Synonym Symbols
C5A; C5AR; C5R1; CD88
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NCBI Protein Information
C5a anaphylatoxin chemotactic receptor 1
UniProt Protein Name
C5a anaphylatoxin chemotactic receptor
UniProt Gene Name
C5AR1 [Similar Products]
UniProt Synonym Gene Names
C5AR; C5R1; C5a-R; C5aR [Similar Products]
UniProt Entry Name
C5AR_HUMAN
UniProt Comments for CD88
C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.
Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: cell surface; basolateral plasma membrane; integral to plasma membrane; apical part of cell; cytoplasmic membrane-bound vesicle; plasma membrane
Molecular Function: complement component C5a receptor activity; complement component C5a binding; C5a anaphylatoxin receptor activity
Biological Process: neutrophil chemotaxis; organ regeneration; sensory perception of chemical stimulus; activation of MAPK activity; apoptosis; response to lipopolysaccharide; chemotaxis; cell proliferation in hindbrain; signal transduction; mRNA transcription from RNA polymerase II promoter; elevation of cytosolic calcium ion concentration; defense response to Gram-positive bacterium; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation
Research Articles on CD88
1. Furthermore, Apigenin reduced the proliferation of human NPC cells triggered by C5a through negative regulation of C5aR/PCAF/STAT3 axis. These might provide a new insight into the function of Apigenin in cancer treatment, and also provide a potential strategy for treating human NPC through inhibition of C5aR expression on cancer cells
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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