DMC, Monoclonal Antibody

Anti -DMC (Dendritic cell and Monocyte Chemokine-like Protein, VEGF Co-regulated Chemokine 1, VCC1)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 18; NC_000018.9 (46567846..46987172, complement). Location: 18q21.1

Monoclonal

IgG2b

7H252

Mouse

Recognizes human DMC.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 

Small volumes of anti-DYM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Dendritic cell and Monocyte Chemokine-like protein (DMC) is a secreted molecule that is closely related to CXCL8/IL-8 and CXCL14/BRAK. DMC is consitutively produced by airway and intestinal epithelium and induces the chemotaxis of monocytes and dendritic cells. It is strongly upregulated in proliferating vascular endothelial cells and some carcinomas. Mature human DMC shares 73% and 71% aa sequence identity with bovine and mouse DMC, respectively.

Antibodies; Abs to Growth Factors, Cytokines

ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Suitable for use in Flow Cytometry, Immunocytochemistry, ELISA and Western Blot.
Dilution: Flow Cytometry: 25ug/ml. 10ul labels 1-5x10e5 A549 cells in a total reaction volume 200ul. For intracellular staining, cells must first be fixed and permeabilized using 4% paraformaldehyde, 0.1% saponin in PBS.
Immunocytochemistry: 10ug/ml detects DMC in A549 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted D8074-90B followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS, BSA.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~10ng/well.
Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.

75,935 Da[Similar Products]

dymeclin

dymeclin; dyggve-Melchior-Clausen syndrome protein

Dymeclin

DYM_HUMAN

This gene encodes a protein which is necessary for normal skeletal development and brain function. Mutations in this gene are associated with two types of recessive osteochondrodysplasia, Dyggve-Melchior-Clausen (DMC) dysplasia and Smith-McCort (SMC) dysplasia, which involve both skeletal defects and mental retardation. [provided by RefSeq, Jul 2008]

DYM: Necessary for correct organization of Golgi apparatus. Involved in bone development. Defects in DYM are the cause of Dyggve-Melchior-Clausen syndrome (DMC). DMC is a rare autosomal recessive disorder characterized by short trunk dwarfism, microcephaly and psychomotor retardation. Electron microscopic study of cutaneous cells of affected patients shows dilated rough endoplasmic reticulum, enlarged and aberrant vacuoles and numerous vesicles. DMC is progressive. Defects in DYM are the cause of Smith-McCort dysplasia (SMC). SMC is a rare autosomal recessive osteochondrodysplasia characterized by short limbs and trunk with barrel-shaped chest. The radiographic phenotype includes platyspondyly, generalized abnormalities of the epiphyses and metaphyses, and a distinctive lacy appearance of the iliac crest, features identical to those of Dyggve-Melchior-Clausen syndrome. Belongs to the dymeclin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: Golgi apparatus; cytoplasm

Molecular Function: protein binding; enzyme binding

Biological Process: Golgi organization and biogenesis

Disease: Dyggve-melchior-clausen Disease; Smith-mccort Dysplasia 1

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