Acetyl CoA Carboxylase 1, Polyclonal Antibody

Anti -Acetyl CoA Carboxylase 1 (ACAC, ACC, ACC1, ACCA, ACC-alpha, Acetyl-CoA carboxylase alpha)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 17; NC_000017.10 (35441927..35766902, complement). Location: 17q21

Polyclonal

IgG

Rabbit

Recongnizes endogenous levels of human total Acetyl CoA Carboxylase 1 protein. Species crossreactivity: mouse and rat.

Affinity Purified
Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-ACACA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACCalpha (ACC1) is the predominant isoform found in liver, adipocytes and mammary gland, while the 280kD ACCbeta (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).

Antibodies; Abs to Enzymes

Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Suitable for use in Western Blot, Immunofluorescence, and Immunoprecipitation.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunofluorescence (IC): 1:800
Immunoprecipitation: 1:50

265,554 Da[Similar Products]

acetyl-CoA carboxylase alpha

acetyl-CoA carboxylase 1; ACC-alpha; OTTHUMP00000164069; OTTHUMP00000164070; OTTHUMP00000164076; acetyl-Coenzyme A carboxylase alpha

Acetyl-CoA carboxylase 1

ACAC; ACC1; ACCA  [Similar Products]

ACACA_HUMAN

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq]

ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity.

Protein type: Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - propanoate; Ligase; Lipid Metabolism - fatty acid biosynthesis; EC 6.4.1.2; EC 6.3.4.14

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: mitochondrion; cytoplasm; nucleolus; cytosol; actin cytoskeleton

Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding

Biological Process: vitamin metabolic process; acetyl-CoA metabolic process; positive regulation of cellular metabolic process; lipid homeostasis; triacylglycerol biosynthetic process; cellular lipid metabolic process; carnitine shuttle; biotin metabolic process; protein homotetramerization; multicellular organismal protein metabolic process; tissue homeostasis; energy reserve metabolic process; water-soluble vitamin metabolic process; fatty acid biosynthetic process

Disease: Acetyl-coa Carboxylase Deficiency

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