Phospho-rat TSC1 (Y297), Polyclonal Antibody

Hamartin; Tuberous sclerosis 1 protein homolog; Tsc1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This rat TSC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y297 of rat TSC1.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.37 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-Tsc1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity).

Phospho Antibodies

Dot Blot (DB), ELISA (EIA)

DB~~1:500

Dot blot analysis of rat TSC1 Antibody (Phospho Y297) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.

129022

tuberous sclerosis 1

hamartin

Hamartin

TSC1_RAT

TSC1: tuberous sclerosis protein 1 (TSC1). May have tumor suppressor activity. Together with tuberin (TSC2), inhibits mammalian target of rapamycin (mTOR)-mediated signaling to eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and ribosomal protein S6 kinase. Regulated by the phosphatidylinositol 3-kinase/Akt pathway and phosphorylation of tuberin. Phosphorylated by cyclin-dependent kinase 1/cyclin B during the cell cycle.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Cellular Component: TSC1-TSC2 complex; growth cone; cytoskeleton; protein complex; intracellular membrane-bound organelle; membrane; lamellipodium; cytoplasm; plasma membrane; actin filament; cell cortex; cytosol

Molecular Function: chaperone binding; GTPase activating protein binding; protein N-terminus binding; GTPase regulator activity

Biological Process: myelination; regulation of cell cycle; protein heterooligomerization; regulation of cell-matrix adhesion; cell-matrix adhesion; negative regulation of insulin receptor signaling pathway; negative regulation of cell size; regulation of phosphoprotein phosphatase activity; cardiac muscle cell differentiation; response to insulin stimulus; regulation of translation; negative regulation of cell proliferation; positive regulation of focal adhesion formation; cell projection organization and biogenesis; synapse organization and biogenesis; kidney development; potassium ion transport; nervous system development; negative regulation of TOR signaling pathway; protein stabilization; hippocampus development; rRNA export from nucleus; regulation of actin cytoskeleton organization and biogenesis; regulation of stress fiber formation; glucose import; negative regulation of translation; neural tube closure; regulation of focal adhesion formation; cerebral cortex development; regulation of protein kinase activity

Inoue, H., et al. Biosci. Biotechnol. Biochem. 73(11):2488-2493(2009)
Di Nardo, A., et al. J. Neurosci. 29(18):5926-5937(2009)
Chen, P., et al. Exp. Mol. Pathol. 86(2):101-107(2009)
Momose, S., et al. Biochem. Biophys. Res. Commun. 356(3):693-698(2007)
Goncharova, E., et al. J. Cell Biol. 167(6):1171-1182(2004)

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