Full Product Name
NF-L Polyclonal Antibody
Product Synonym Names
NEFL; NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein
Product Gene Name
anti-NF-L antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Antigen Affinity Purified
Concentration
1 mg/ml (lot specific)
Immunogen
Synthesized peptide derived from the C-terminal region of human NF-L.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-NF-L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-NF-L antibody
Stem cells; Neuroscience
Applications Tested/Suitable for anti-NF-L antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-NF-L antibody
WB: 1:500-1:2000, ELISA: 1:5000-1:20000
Western Blot (WB) of anti-NF-L antibody
Western Blot analysis of Jurkat cells with NF-L Polyclonal Antibody.
Immunohistochemistry (IHC) of anti-NF-L antibody
Immunohistochemistry of paraffin-embedded Rat brain tissue using NF-L Polyclonal Antibody at dilution of 1:100.
NCBI/Uniprot data below describe general gene information for NF-L. It may not necessarily be applicable to this product.
NCBI Accession #
NP_006149.2
[Other Products]
NCBI GenBank Nucleotide #
NM_006158.4
[Other Products]
UniProt Secondary Accession #
Q16154; Q8IU72; B9ZVN2[Other Products]
UniProt Related Accession #
P07196[Other Products]
Molecular Weight
Calculated MW: 62
Observed MW: 61
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament, light polypeptide
NCBI Official Symbol
NEFL [Similar Products]
NCBI Official Synonym Symbols
NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110
[Similar Products]
NCBI Protein Information
neurofilament light polypeptide
UniProt Protein Name
Neurofilament light polypeptide
UniProt Synonym Protein Names
68 kDa neurofilament protein; Neurofilament triplet L protein
UniProt Gene Name
NEFL [Similar Products]
UniProt Synonym Gene Names
NF68; NFL; NF-L [Similar Products]
UniProt Entry Name
NFL_HUMAN
NCBI Summary for NF-L
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
UniProt Comments for NF-L
NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F).
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 8p21
Cellular Component: axon; cytoplasm; cytosol; growth cone; myelin sheath; neurofilament; TSC1-TSC2 complex
Molecular Function: identical protein binding; phospholipase binding; protein binding; protein binding, bridging; protein C-terminus binding; protein domain specific binding; protein heterodimerization activity; structural constituent of cytoskeleton
Biological Process: activation of MAPKK activity; anterograde axon cargo transport; axon guidance; axon regeneration in the peripheral nervous system; axon transport of mitochondrion; cerebral cortex development; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; hippocampus development; innate immune response; insulin receptor signaling pathway; intermediate filament organization; intermediate filament polymerization and/or depolymerization; MAPKKK cascade; microtubule cytoskeleton organization and biogenesis; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; neurofilament bundle assembly; neuromuscular process controlling balance; positive regulation of axonogenesis; protein polymerization; Ras protein signal transduction; regulation of axon diameter; response to corticosterone stimulus; response to toxin; retrograde axon cargo transport; small GTPase mediated signal transduction; spinal cord development; synaptic transmission; vascular endothelial growth factor receptor signaling pathway
Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f
Research Articles on NF-L
1. Suggest monitoring the serum level of antibodies against the NF-L chain as a predictive biomarker of treatment response in multiple sclerosis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
