Product Name
BCMA (CD269), Recombinant Protein
Full Product Name
Human BCMA (CD269) Recombinant
Product Synonym Names
cd269; bcma; TNFRSF17
Product Gene Name
BCMA recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MLQMAGQCSQ NEYFDSLLHA CIPCQLRCSS NTPPLTCQRY CNASVTNSVK GTNAILWTCL
3D Structure
ModBase 3D Structure for Q02223
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant BCMA was lyophilized from a 0.2 um filtered 30% acetonitrile, 0.1% TFA solution pH 7.5.
Host Note
Optimized DNA sequence encoding Human BCMA extracellular domain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant BCMA is a disulfide-linked monomer protein consisting of 54 amino acid residue subunits, and migrates as an approximately 6kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this product can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of BCMA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BCMA recombinant protein
BCMA (B cell maturation) is a nonglycosylated integral membrane type I protein that is preferentially expressed in mature B lymphocytes.The N-terminal part of both mouse and human proteins contains a conserved six- cysteine motif. BLyS binds to three receptors of the TNF receptor family (B cell maturation antigen BCMA, TACI, and BAFF-R), all of which are expressed by mature B cells.
Product Categories/Family for BCMA recombinant protein
Cytokines
NCBI/Uniprot data below describe general gene information for BCMA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001183.2
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NCBI GenBank Nucleotide #
NM_001192.2
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UniProt Primary Accession #
Q02223
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UniProt Related Accession #
Q02223; Q13808; Q2TQ40; Q6PE46[Other Products]
Molecular Weight
20,165 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 17
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 17
UniProt Synonym Protein Names
B-cell maturation protein
UniProt Gene Name
TNFRSF17 [Similar Products]
UniProt Synonym Gene Names
BCM; BCMA [Similar Products]
UniProt Entry Name
TNR17_HUMAN
NCBI Summary for BCMA
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. [provided by RefSeq, Jul 2008]
UniProt Comments for BCMA
TNFRSF17: Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK. A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
Protein type: Membrane protein, integral; Oncoprotein; Receptor, misc.
Chromosomal Location of Human Ortholog: 16p13.1
Cellular Component: integral to membrane; plasma membrane; endomembrane system
Molecular Function: receptor activity
Biological Process: cell proliferation; immune system process; multicellular organismal development; signal transduction
Product References and Citations for BCMA recombinant protein
• autoantibody-producing rp105– b cells, from patients with systemic lupus erythematosus, showed more preferential expression of BCMA compared with baff-r than normal subjects .Rheumatology, Jan 2010; 10.1093/rheumatology/kep437.
• adipocytes as immune cells: differential expression of tweak, baff, and april and their receptors (fn14, baff-r, taci, and BCMA) at different stages of normal and pathological adipose tissue development .J. Immunol., Nov 2009; 183: 5948 - 5956.
• activation of b-cell maturation antigen (BCMA) on human multiple myeloma cells by a proliferation-inducing ligand (april) promotes myeloma cell function in the bone marrow microenvironment. .Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 1503.
• the rgel/blys fusion toxin specifically targets malignant b cells expressing the blys receptors baff-r, taci, and BCMA .Mol. Cancer Ther., Feb 2007; 6: 460 - 470.
• hodgkin lymphoma cells express taci and BCMA receptors and generate survival and proliferation signals in response to baff and april .Blood, Jan 2007; 109: 729 - 739.
Precautions
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Disclaimer
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