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3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), RTU ELISA Kit

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产品名称: 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), RTU ELISA Kit
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简单介绍

3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), RTU ELISA Kit


3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), RTU ELISA Kit  的详细介绍
Product Name

3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR), RTU ELISA Kit

Full Product Name

Rat 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) ELISA Kit

Product Synonym Names
HMG-CoA Reductase; Hydroxymethylglutaryl-CoA Reductase
Product Gene Name

HMGCR rtu elisa kit

[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Sample Manual Insert
Download Sample PDF Manual View Sample PDF Manual
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for P51639
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Tissue Homogenates, Cell Lysates or Other Biological Fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.134ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of HMGCR rtu elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
HMGCR rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMGCR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HMGCR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HMGCR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HMGCR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of HMGCR rtu elisa kit
HMGCR rtu elisa kit Typical Testing Data/Standard Curve (for reference only) image
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NCBI/Uniprot data below describe general gene information for HMGCR. It may not necessarily be applicable to this product.
NCBI GI #
40538852
NCBI GeneID
25675
NCBI Accession #
NP_037266.2 [Other Products]
NCBI GenBank Nucleotide #
NM_013134.2 [Other Products]
UniProt Primary Accession #
P51639 [Other Products]
UniProt Secondary Accession #
Q64601; Q6P2A6[Other Products]
UniProt Related Accession #
P51639[Other Products]
Molecular Weight
96,688 Da
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NCBI Official Full Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase
NCBI Official Synonym Full Names
3-hydroxy-3-methylglutaryl-CoA reductase
NCBI Official Symbol
Hmgcr  [Similar Products]
NCBI Official Synonym Symbols
3H3M
  [Similar Products]
NCBI Protein Information
3-hydroxy-3-methylglutaryl-coenzyme A reductase
UniProt Protein Name
3-hydroxy-3-methylglutaryl-coenzyme A reductase
UniProt Gene Name
Hmgcr  [Similar Products]
UniProt Synonym Gene Names
HMG-CoA reductase  [Similar Products]
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NCBI Summary for HMGCR
enzyme involved in mevalonate synthesis [RGD, Feb 2006]
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UniProt Comments for HMGCR
Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.
Research Articles on HMGCR
1. findings demonstrate for the first time that strain-dependent sex difference in serum Total Cholesterol level between F344 and SD rats is, at least in part, related to difference in the IL-1alpha-mediated HMGR gene expression level in the liver.
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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