Product Name
NLRP7, siRNA
Full Product Name
NLRP7 siRNA (Human)
Product Synonym Names
NALP7; NOD12; PYPAF3; NACHT. LRR and PYD domains-containing protein 7; Nucleotide-binding oligomerization domain protein 12; PYRIN-containing APAF1-like protein 3
Product Gene Name
NLRP7 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8WX94
Specificity
NLRP7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NLRP7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NLRP7 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NLRP7 sirna
siRNA to inhibit NLRP7 expression using RNA interference
Applications Tested/Suitable for NLRP7 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NLRP7. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001120727.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001127255.1
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UniProt Primary Accession #
Q8WX94
[Other Products]
UniProt Secondary Accession #
Q32MH8; Q7RTR1; E9PE16[Other Products]
UniProt Related Accession #
Q8WX94[Other Products]
Molecular Weight
118,303 Da
NCBI Official Full Name
NACHT, LRR and PYD domains-containing protein 7 isoform 3
NCBI Official Synonym Full Names
NLR family, pyrin domain containing 7
NCBI Official Symbol
NLRP7 [Similar Products]
NCBI Official Synonym Symbols
HYDM; PAN7; NALP7; NOD12; PYPAF3; CLR19.4
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NCBI Protein Information
NACHT, LRR and PYD domains-containing protein 7
UniProt Protein Name
NACHT, LRR and PYD domains-containing protein 7
UniProt Synonym Protein Names
Nucleotide-binding oligomerization domain protein 12; PYRIN-containing APAF1-like protein 3
Protein Family
NACHT, LRR and PYD domains-containing protein
UniProt Gene Name
NLRP7 [Similar Products]
UniProt Synonym Gene Names
NALP7; NOD12; PYPAF3 [Similar Products]
UniProt Entry Name
NALP7_HUMAN
NCBI Summary for NLRP7
This gene encodes a member of the NACHT, leucine rich repeat, and PYD containing (NLRP) protein family. It has an N-terminal pyrin domain, followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. NLRP proteins are implicated in the activation of proinflammatory caspases through multiprotein complexes called inflammasomes. This gene may act as a feedback regulator of caspase-1-dependent interleukin 1-beta secretion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for NLRP7
NLRP7: Inhibits CASP1/caspase-1-dependent IL1B secretion. Defects in NLRP7 are the cause of hydatidiform mole recurrent type 1 (HYDM1). A disorder characterized by excessive trophoblast development and the presence of a growing mass of tissue inside the uterus at the beginning of a pregnancy. It leads to abnormal pregnancies with no embryo, and cystic degeneration of the chorionic villi. Belongs to the NLRP family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 19q13.42
Molecular Function: ATP binding
Disease: Hydatidiform Mole, Recurrent, 1
Research Articles on NLRP7
1. NLRP7 thus appears to function in chromatin reprogramming and DNA methylation in the germline or early embryonic development.NLRP7 affects trophoblast lineage differentiation, binds to overexpressed YY1 and alters CpG methylation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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