Product Name
Apaxin (APTX), ELISA Kit
Full Product Name
Monkey Apaxin (APTX) ELISA Kit
Product Gene Name
APTX elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
Species Reactivity
Monkey
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Preparation and Storage
Store all reagents at 2-8 degree C.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of APTX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for APTX purchase
MBS7223727 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Apaxin (APTX) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing APTX. The ELISA analytical biochemical technique of the MBS7223727 kit is based on APTX antibody-APTX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect APTX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, APTX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Product Categories/Family for APTX elisa kit
Epigenetics and Nuclear Signaling
NCBI/Uniprot data below describe general gene information for APTX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001182181.1
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NCBI GenBank Nucleotide #
NM_001195252.1
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UniProt Secondary Accession #
Q0P662; Q5T781; Q5T782; Q5T784; Q6JV81; Q6JV82; Q6JV85; Q7Z2F3; A8MTN4; D3DRK9; D3DRL0[Other Products]
UniProt Related Accession #
Q7Z2E3[Other Products]
Molecular Weight
21,389 Da
NCBI Official Full Name
aprataxin isoform i
NCBI Official Synonym Full Names
aprataxin
NCBI Official Symbol
APTX [Similar Products]
NCBI Official Synonym Symbols
AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT
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NCBI Protein Information
aprataxin; forkhead-associated domain histidine triad-like protein
UniProt Protein Name
Aprataxin
UniProt Synonym Protein Names
Forkhead-associated domain histidine triad-like protein; FHA-HIT
UniProt Gene Name
APTX [Similar Products]
UniProt Synonym Gene Names
AXA1; FHA-HIT [Similar Products]
UniProt Entry Name
APTX_HUMAN
NCBI Summary for APTX
This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Aug 2010]
UniProt Comments for APTX
APTX: DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non- ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'- monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Defects in APTX are the cause of ataxia-oculomotor apraxia syndrome (AOA). AOA is an autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy. 13 isoforms of the human protein are produced by alternative splicing.
Protein type: Nucleolus; Phosphatase (non-protein); DNA repair, damage; RNA-binding; EC 3.-.-.-; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 9p13.3
Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; nucleolus; chromatin; nucleus
Molecular Function: protein binding; DNA 5'-adenosine monophosphate hydrolase activity; phosphoglycolate phosphatase activity; polynucleotide 3'-phosphatase activity; metal ion binding; double-stranded RNA binding; double-stranded DNA binding; damaged DNA binding; phosphoprotein binding; protein N-terminus binding; chromatin binding
Biological Process: single strand break repair; response to hydrogen peroxide; dephosphorylation; double-strand break repair; regulation of protein stability; DNA catabolic process, exonucleolytic; response to DNA damage stimulus; DNA ligation
Disease: Ataxia, Early-onset, With Oculomotor Apraxia And Hypoalbuminemia
Research Articles on APTX
1. Structure-function studies of human APTX-RNA-DNA-AMP-Zn complexes define a mechanism for detecting and reversing adenylation at RNA-DNA junctions
Precautions
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Disclaimer
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