1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1 (PLCG1), ELI

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of PLCG1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7246536 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1 (PLCG1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCG1. The ELISA analytical biochemical technique of the MBS7246536 kit is based on PLCG1 antibody-PLCG1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCG1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCG1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Signal Transduction

148,548 Da

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1

PLC-gamma-1  [Similar Products]

PLCG1_RAT

PLCG1: a calcium dependent phosphatidylinositol-specific phospholipase C. The activated enzyme produces the second messenger molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylated and activated by tyrosine kinases in response to signaling through a variety of growth factor receptors and immune system receptors.

Protein type: Motility/polarity/chemotaxis; Phospholipase; Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11

Cellular Component: signalosome; ruffle; neuron projection; cell projection; lamellipodium; cytoplasm; plasma membrane; intercellular junction; cytosol

Molecular Function: signal transducer activity; protein binding; glutamate receptor binding; neurotrophin TRKA receptor binding; phosphoprotein binding; calcium ion binding; receptor tyrosine kinase binding; protein kinase binding; phosphoinositide phospholipase C activity; insulin receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to gravity; cell migration; phospholipid catabolic process; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; protein secretion; calcium-mediated signaling; response to morphine; response to organic nitrogen; signal transduction; T cell receptor signaling pathway; positive regulation of angiogenesis; response to hydrogen peroxide; inositol trisphosphate biosynthetic process; calcium ion transport; positive regulation of release of sequestered calcium ion into cytosol

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