ADAM metallopeptidase domain 33, ELISA Kit

Mouse Disintegrin and metalloproteinase domain-containing protein 33 (ADAM33) ELISA kit; RP5-964F7.2; C20orf153; DJ964F7.1; DKFZp434K0521; FLJ35308; FLJ36751; MGC149823; MGC71889; a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; disintegrin and reprolys; ADAM metallopeptidase domain 33

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of mouse ADAM33. No significant cross-reactivity or interference between mouse ADAM33 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ADAM33 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS922898 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the ADAM metallopeptidase domain 33 (ADAM33) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADAM33. The ELISA analytical biochemical technique of the MBS922898 kit is based on ADAM33 antibody-ADAM33 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADAM33 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADAM33. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM33 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM33 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM33 bound in the initial step. The color development is stopped and the intensity of the color is measured.

86,971 Da

a disintegrin and metallopeptidase domain 33

disintegrin and metalloproteinase domain-containing protein 33; ADAM 33; a disintegrin and metalloprotease domain 33

Disintegrin and metalloproteinase domain-containing protein 33

ADAM 33  [Similar Products]

ADA33_MOUSE

ADAM33: Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA). The most common chronic disease affecting children and young *****s. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.4.24.-; Protease

Cellular Component: membrane; integral to membrane

Molecular Function: peptidase activity; hydrolase activity; zinc ion binding; metallopeptidase activity; metal ion binding; metalloendopeptidase activity

Biological Process: proteolysis; regulation of cytokine biosynthetic process; regulation of cell proliferation

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.