CFC1, Polyclonal Antibody

HTX2; CFC1B; DTGA2; CRYPTIC

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

The antibody detects endogenous levels of total CFC1 protein.

Antigen Affinity Purification

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

1.1 mg/ml (lot specific)

Store at -20 degree C

Small volumes of anti-CFC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family, which are involved in signalling during embryonic development.Proteins in this family share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. The protein encoded by this gene is necessary for patterning the left-right embryonic axis.

Total protein Ab

Western Blot (WB), Immunohistochemistry (IHC)

Western Blot: 1:200-1:1000
Immunohistochemistry: 1:30-1:150

Gel: 12%SDS-PAGE Lysate: 40 μ g Lane: Human fetal intestines tissue Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 2 minutes

Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/20.

Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/20.

25kDa

cripto, FRL-1, cryptic family 1

cryptic protein

Cryptic protein

CFC1_HUMAN

CFC1: NODAL coreceptor involved in the correct establishment of the left-right axis. May play a role in mesoderm and/or neural patterning during gastrulation. Defects in CFC1 are the cause of visceral heterotaxy autosomal type 2 (HTX2). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects including cardiac malformations and situs inversus or situs ambiguus. Defects in CFC1 are a cause of transposition of the great arteries dextro-looped type 2 (DTGA2). A congenital heart defect consisting of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. Patients often have atrial and/or ventricular septal defects or other types of shunting that allow some mixing between the circulations in order to support life minimally, but surgical intervention is always required. Defects in CFC1 are a cause of conotruncal heart malformations (CTHM). CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 2q21.1

Disease: Conotruncal Heart Malformations; Heterotaxy, Visceral, 2, Autosomal; Transposition Of The Great Arteries, Dextro-looped 2

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