MAFA, Polyclonal Antibody

Transcription factor MafA; Pancreatic beta-cell-specific transcriptional activator; Transcription factor RIPE3b1; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This MAFA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human MAFA.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-MAFA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MAFA is a transcription factor that binds RIPE3b, a
conserved enhancer element that regulates pancreatic beta
cell-specific expression of the insulin gene (INS; MIM 176730)
(Olbrot et al., 2002 [PubMed 12011435]).

Cancer; Signal Transduction

Western Blot (WB), ELISA (EIA)

WB~~1:1000

MAFA Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAFA antibody detected the MAFA protein (arrow).

v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A

transcription factor MafA

Transcription factor MafA

MAFA_HUMAN

MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008]

MAFA: Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds DNA as a homodimer (Probable). Interacts with NEUROD1, PCAF and PDX1. Up-regulated by glucose. Belongs to the bZIP family. Maf subfamily.

Protein type: Tumor suppressor; DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; insulin secretion; response to glucose stimulus; positive regulation of transcription from RNA polymerase II promoter; nitric oxide mediated signal transduction

Lecoin, L., et al. Dev Neurobiol 70(7):485-497(2010)
Guo, S., et al. J. Biol. Chem. 285(17):12655-12661(2010)
Kondo, T., et al. Mol. Endocrinol. 23(8):1281-1290(2009)
Kaneto, H., et al. Adv. Drug Deliv. Rev. 61 (7-8), 489-496 (2009) :
Garin, I., et al. Diabet. Med. 26(7):746-748(2009)

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.