Product Name
ACVRL1, siRNA
Full Product Name
ACVRL1 siRNA (Mouse)
Product Synonym Names
ACVRLK1; ALK-1; Serine/threonine-protein kinase receptor R3; SKR3; Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I
Product Gene Name
ACVRL1 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q61288
Specificity
ACVRL1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ACVRL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ACVRL1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ACVRL1 sirna
siRNA to inhibit ACVRL1 expression using RNA interference
Applications Tested/Suitable for ACVRL1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ACVRL1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001264186.1
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NCBI GenBank Nucleotide #
NM_001277257.1
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UniProt Primary Accession #
Q61288
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UniProt Secondary Accession #
Q61289; Q91YR0[Other Products]
UniProt Related Accession #
Q61288[Other Products]
Molecular Weight
56,519 Da
NCBI Official Full Name
serine/threonine-protein kinase receptor R3 isoform a
NCBI Official Synonym Full Names
activin A receptor, type II-like 1
NCBI Official Symbol
Acvrl1 [Similar Products]
NCBI Official Synonym Symbols
Alk1; Acvrlk1; AI115505; AI427544
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NCBI Protein Information
serine/threonine-protein kinase receptor R3
UniProt Protein Name
Serine/threonine-protein kinase receptor R3
UniProt Synonym Protein Names
Activin receptor-like kinase 1; ALK-1; TGF-B superfamily receptor type I; TSR-I
Protein Family
Serine/threonine-protein kinase receptor
UniProt Gene Name
Acvrl1 [Similar Products]
UniProt Synonym Gene Names
Acvrlk1; Alk-1; SKR3; ALK-1; TSR-I [Similar Products]
UniProt Entry Name
ACVL1_MOUSE
UniProt Comments for ACVRL1
ALK1: a kinase of the STKR family. A type I cell-surface receptor for the TGF-beta superfamily of ligands including Activin A. Highly expressed in human placenta and lung. Deficiency causes hemorrhagic telangiectasia type 2 also known as Rendu-Osler-Weber syndrome 2.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (receptor); EC 2.7.11.30; Protein kinase, TKL; Membrane protein, integral; TKL group; STKR family; Type1 subfamily
Cellular Component: cell surface; membrane; cell soma; integral to plasma membrane; dendrite; integral to membrane
Molecular Function: metal ion binding; nucleotide binding; protein kinase binding; transmembrane receptor protein serine/threonine kinase activity; protein kinase activity; transforming growth factor beta receptor activity; transferase activity; protein serine/threonine kinase activity; protein binding; activin receptor activity, type I; transforming growth factor beta receptor activity, type I; transforming growth factor beta binding; transferase activity, transferring phosphorus-containing groups; activin binding; kinase activity; SMAD binding; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: protein heterooligomerization; blood circulation; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; negative regulation of cell adhesion; signal transduction; protein amino acid phosphorylation; BMP signaling pathway; negative regulation of cell proliferation; positive regulation of endothelial cell differentiation; regulation of transcription, DNA-dependent; negative regulation of endothelial cell differentiation; transforming growth factor beta receptor signaling pathway; regulation of blood pressure; lymphangiogenesis; transmembrane receptor protein serine/threonine kinase signaling pathway; angiogenesis; negative regulation of cell migration; positive regulation of BMP signaling pathway; in utero embryonic development; negative regulation of focal adhesion formation; negative regulation of blood vessel endothelial cell migration; endothelial cell activation; positive regulation of angiogenesis; negative regulation of endothelial cell proliferation; blood vessel morphogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; blood vessel remodeling; negative regulation of cell growth; phosphorylation; wound healing, spreading of epidermal cells
Research Articles on ACVRL1
1. during development, endothelial Acvrl1 plays an essential role to regulate endothelial cell proliferation and arterial identity during angiogenesis, whilst in ***** life endothelial Acvrl1 is required to maintain vascular integrity.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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