Product Name
MFSD8, siRNA
Full Product Name
MFSD8 siRNA (Human)
Product Synonym Names
CLN7; Major facilitator superfamily domain-containing protein 8; Ceroid-lipofuscinosis neuronal protein 7
Product Gene Name
MFSD8 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8NHS3
Specificity
MFSD8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MFSD8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MFSD8 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MFSD8 sirna
siRNA to inhibit MFSD8 expression using RNA interference
Applications Tested/Suitable for MFSD8 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MFSD8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_689991.1
[Other Products]
NCBI GenBank Nucleotide #
NM_152778.2
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UniProt Primary Accession #
Q8NHS3
[Other Products]
UniProt Secondary Accession #
Q8N2P3; B2RDM1; B7Z205[Other Products]
UniProt Related Accession #
Q8NHS3[Other Products]
Molecular Weight
17,092 Da
NCBI Official Full Name
major facilitator superfamily domain-containing protein 8
NCBI Official Synonym Full Names
major facilitator superfamily domain containing 8
NCBI Official Symbol
MFSD8 [Similar Products]
NCBI Official Synonym Symbols
CCMD; CLN7
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NCBI Protein Information
major facilitator superfamily domain-containing protein 8
UniProt Protein Name
Major facilitator superfamily domain-containing protein 8
UniProt Synonym Protein Names
Ceroid-lipofuscinosis neuronal protein 7
Protein Family
Major facilitator superfamily domain-containing protein
UniProt Gene Name
MFSD8 [Similar Products]
UniProt Synonym Gene Names
CLN7 [Similar Products]
UniProt Entry Name
MFSD8_HUMAN
NCBI Summary for MFSD8
This gene encodes a ubiquitous integral membrane protein that contains a transporter domain and a major facilitator superfamily (MFS) domain. Other members of the major facilitator superfamily transport small solutes through chemiosmotic ion gradients. The substrate transported by this protein is unknown. The protein likely localizes to lysosomal membranes. Mutations in this gene are correlated with a variant form of late infantile-onset neuronal ceroid lipofuscinoses (vLINCL). [provided by RefSeq, Oct 2008]
UniProt Comments for MFSD8
MFSD8: May be a carrier that transport small solutes by using chemiosmotic ion gradients (Potential). Defects in MFSD8 are the cause of neuronal ceroid lipofuscinosis type 7 (CLN7). A form of late infantile neuronal ceroid lipofuscinosis. CNL are a clinically and genetically heterogeneous group of neurodegenerative disorders characterized by the intracellular accumulation of autofluorescent lipopigment storage material in different patterns ultrastructurally. The patterns most often observed CLN7 are mixed combinations of granular, curvilinear, fingerprint, and rectilinear profiles. The clinical course includes progressive dementia, seizures, and progressive visual failure. Belongs to the major facilitator superfamily.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q28.2
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; lysosomal membrane; integral to membrane
Biological Process: lysosome organization and biogenesis; transmembrane transport
Disease: Ceroid Lipofuscinosis, Neuronal, 7; Macular Dystrophy With Central Cone Involvement
Research Articles on MFSD8
1. A (p.Gly158Ser), segregates with the disease phenotype in variant late infantile neuronal ceroid lipofuscinosis.">A mutation in MFSD8, c.472G>A (p.Gly158Ser), segregates with the disease phenotype in variant late infantile neuronal ceroid lipofuscinosis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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