D-3-phosphoglycerate dehydrogenase, Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (120254419..120286849). Location: 1p12

E Coli

>=90%(SDS-PAGE) (lot specific)

Liquid containing glycerol

This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.

Sterile filter available upon request.

Low endotoxin available upon request.

Store at -20 degree C. For extended storage, conserve at -20 or -80 degree C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Small volumes of PHGDH recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH.

(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)

63kD [Similar Products]

phosphoglycerate dehydrogenase

D-3-phosphoglycerate dehydrogenase; OTTHUMP00000013926; OTTHUMP00000013927

D-3-phosphoglycerate dehydrogenase

PGDH3  [Similar Products]

SERA_HUMAN

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM, Apr 2004]

Catalytic activity: 3-phospho-D-glycerate + NAD+ = 3-phosphonooxypyruvate + NADH. Ref.102-hydroxyglutarate + NAD+ = 2-oxoglutarate + NADH. Ref.10Pathway: Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.Subunit structure: Homotetramer By similarity.Induction: Induced by 17-beta-estradiol (estrogenic ligand) and 4-hydroxytamoxifen (agonist/antagonist ligand). Positively regulated by the transcription factors SP1 and NF-Y. Ref.11 Ref.12Involvement in disease: Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures.Sequence similarities: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.Biophysicochemical propertiesKinetic parameters:KM=21.6 µM for 3-phosphohydroxypyruvate Ref.16Vmax=35 nmol/min/mg enzyme with 3-phosphohydroxypyruvate as substrate (in patient-derived fibroblasts)Vmax=168 nmol/min/mg enzyme with 3-phosphohydroxypyruvate as substrate (in 3-PGDH overexpressed cells)

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