5, 10-methylenetetrahydrofolate reductase (NADPH), ELISA Kit

Human Methylenetetrahydrofolate reductase (MTHFR) ELISA kit; 5; 10-methylenetetrahydrofolate reductase; OTTHUMP00000002367; 5; 10-methylenetetrahydrofolate reductase (NADPH)

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of human MTHFR. No significant cross-reactivity or interference between human MTHFR and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MTHFR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS923196 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5, 10-methylenetetrahydrofolate reductase (NADPH) (MTHFR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MTHFR. The ELISA analytical biochemical technique of the MBS923196 kit is based on MTHFR antibody-MTHFR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MTHFR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MTHFR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MTHFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MTHFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTHFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTHFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

74,597 Da

methylenetetrahydrofolate reductase (NAD(P)H)

methylenetetrahydrofolate reductase; 5,10-methylenetetrahydrofolate reductase (NADPH)

Methylenetetrahydrofolate reductase

MTHR_HUMAN

The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq, Oct 2009]

MTHFR: Catalyzes the conversion of 5,10- methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co- substrate for homocysteine remethylation to methionine. Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD). MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia, developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders. Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Belongs to the methylenetetrahydrofolate reductase family.

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Energy Metabolism - methane; Oxidoreductase; EC 1.5.1.20

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: neuron projection; cytosol

Molecular Function: FAD binding; NADP binding; methylenetetrahydrofolate reductase (NADPH) activity

Biological Process: response to drug; amino acid metabolic process; vitamin metabolic process; methionine biosynthetic process; blood circulation; response to folic acid; response to vitamin B2; response to hypoxia; S-adenosylmethionine metabolic process; homocysteine metabolic process; one-carbon compound metabolic process; folic acid metabolic process; water-soluble vitamin metabolic process

Disease: Schizophrenia; Thrombophilia Due To Thrombin Defect; Neural Tube Defects, Folate-sensitive; Homocystinuria Due To Deficiency Of N(5,10)-methylenetetrahydrofolate Reductase Activity

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.