Full Product Name
MRE11 (Phospho-Ser264) Antibody
Product Synonym Names
Double-strand break repair protein MRE11A; HNGS1; MRE11; MRE11 homolog 1; MRE11A; MmMRE11A
Product Gene Name
anti-MRE11 antibody
[Similar Products]
Product Synonym Gene Name
MRE11A[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P49959
Species Reactivity
Human, Mouse, Rat
Specificity
MRE11 (Phospho-Ser264) Antibody detects endogenous levels of MRE11 only when phosphorylated at Ser264.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Phospho
Modification Sites
H:S264; M:S264; R:S264
Immunogen
The antiserum was produced against synthesized peptide derived from human MRE11 around the phosphorylation site of Ser264.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-MRE11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MRE11 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MRE11 antibody
WB: 1:500~1:1000
ELISA: 1:1000
Western Blot (WB) of anti-MRE11 antibody
Western blot analysis of lysates from NIH/3T3 cells treated with forskolin 40nM 30', using MRE11 (Phospho-Ser264) Antibody. The lane on the right is blocked with the phospho peptide.
NCBI/Uniprot data below describe general gene information for MRE11. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005581.2
[Other Products]
NCBI GenBank Nucleotide #
NM_005590.3
[Other Products]
UniProt Primary Accession #
P49959
[Other Products]
UniProt Secondary Accession #
O43475; B3KTC7[Other Products]
UniProt Related Accession #
P49959[Other Products]
NCBI Official Full Name
double-strand break repair protein MRE11 isoform 2
NCBI Official Synonym Full Names
MRE11 homolog, double strand break repair nuclease
NCBI Official Symbol
MRE11 [Similar Products]
NCBI Official Synonym Symbols
ATLD; HNGS1; MRE11A; MRE11B
[Similar Products]
NCBI Protein Information
double-strand break repair protein MRE11; double-strand break repair protein MRE11A
UniProt Protein Name
Double-strand break repair protein MRE11
UniProt Synonym Protein Names
Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A
Protein Family
Double-strand break repair protein
UniProt Gene Name
MRE11 [Similar Products]
UniProt Synonym Gene Names
MRE11 homolog 1; MRE11 homolog A [Similar Products]
NCBI Summary for MRE11
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for MRE11
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
Research Articles on MRE11
1. Low MRE11 expression is associated with low-grade epithelial ovarian cancer.
Precautions
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Disclaimer
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