Full Product Name
MRE11 (Ab-264) Antibody
Product Synonym Names
Double-strand break repair protein MRE11A; HNGS1; MRE11; MRE11 homolog 1; MRE11A; MmMRE11A
Product Gene Name
anti-MRE11 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P49959
Specificity
MRE11 (Ab-264) antibody detects endogenous levels of total MRE11 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human MRE11 around the phosphorylation site of serine 264(Y-I-SP-Q-P).
Preparation and Storage
Store at-20 degree C for 1 year.
Other Notes
Small volumes of anti-MRE11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MRE11 antibody
Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells
Applications Tested/Suitable for anti-MRE11 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-MRE11 antibody
WB: 1:500~1:1000
ELISA: 1:20000
Western Blot (WB) of anti-MRE11 antibody
Western blot analysis of extracts from Jurkat cells, treated with UV (15mins), using MRE11 (Ab-264) antibody.
NCBI/Uniprot data below describe general gene information for MRE11. It may not necessarily be applicable to this product.
NCBI Accession #
NP_005581.2
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NCBI GenBank Nucleotide #
NM_005590.3
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UniProt Primary Accession #
P49959
[Other Products]
UniProt Secondary Accession #
O43475; B3KTC7[Other Products]
UniProt Related Accession #
P49959[Other Products]
Molecular Weight
81,070 Da
NCBI Official Full Name
double-strand break repair protein MRE11 isoform 2
NCBI Official Synonym Full Names
MRE11 homolog, double strand break repair nuclease
NCBI Official Symbol
MRE11 [Similar Products]
NCBI Official Synonym Symbols
ATLD; HNGS1; MRE11A; MRE11B
[Similar Products]
NCBI Protein Information
double-strand break repair protein MRE11
UniProt Protein Name
Double-strand break repair protein MRE11
UniProt Synonym Protein Names
Double-strand break repair protein MRE11A; Meiotic recombination 11 homolog 1; MRE11 homolog 1; Meiotic recombination 11 homolog A; MRE11 homolog A
Protein Family
Double-strand break repair protein
UniProt Gene Name
MRE11 [Similar Products]
UniProt Synonym Gene Names
MRE11 homolog 1; MRE11 homolog A [Similar Products]
NCBI Summary for MRE11
This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Comments for MRE11
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).
Product References and Citations for anti-MRE11 antibody
Petrini J.H.J., Genomics 29:80-86(1995). Paull T.T., Mol. Cell 1:969-979(1998). Pitts S.A., Hum. Mol. Genet. 10:1155-1162(2001).
Research Articles on MRE11
1. MRE11A gene polymorphism is associated with colorectal cancer.
Precautions
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Disclaimer
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