5'-AMP-activated protein kinase subunit beta-1, ELISA Kit

5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb; 5'-AMP-activated protein kinase 40 kDa subunit; Prkab1

For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C

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Small volumes of Prkab1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9427693 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5'-AMP-activated protein kinase subunit beta-1 (Prkab1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Prkab1. The ELISA analytical biochemical technique of the MBS9427693 kit is based on Prkab1 antibody-Prkab1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Prkab1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Prkab1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

30,394 Da

protein kinase AMP-activated non-catalytic subunit beta 1

5'-AMP-activated protein kinase subunit beta-1

5'-AMP-activated protein kinase subunit beta-1

AMPK subunit beta-1; AMPKb  [Similar Products]

may facilitate the association of the heterotrimeric AMP-activated protein kinase; may phosphorylate and inactivate enzymes involved in lipid metabolism; may play a role in response to metabolic stress [RGD, Feb 2006]

AMPKB1: a non-catalytic subunit of AMPK, a conserved kinase of the CAMKL family. AMPK is an energy-sensing protein that plays a key role in regulating cellular energy homeostasis. Environmental stress, such as heat shock, nutrient deprivation, hypoxia and ischemia, indirectly activate AMPK by the depletion of cellular ATP and the concomitant rise of ADP and AMP levels. Allosteric activation is achieved primarily by rising ADP levels, and not solely by AMP levels as previously thought. Activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Beta subunits act as scaffolds on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. AMPK-beta1 or -beta2 subunits are required for assembling of AMPK heterotrimers and are important for regulating enzyme activity and cellular localization. AMPK beta1beta2 null mouse muscles reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Phosphorylation by ULK1 and ULK2 inhibits AMPK activity. Hematopoietic AMPKB1 reduces mouse adipose tissue macrophage inflammation and insulin resistance in obesity.

Protein type: Autophagy; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 12q16

Cellular Component: AMP-activated protein kinase complex; nucleus; protein complex

Molecular Function: AMP-activated protein kinase activity; protein binding; protein kinase activity; protein kinase binding

Biological Process: protein amino acid phosphorylation; protein heterooligomerization; regulation of catalytic activity

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