SAR1B, Polyclonal Antibody

GTP-binding protein SAR1b; GTP-binding protein B; GTBPB; SAR1B; SARA2; SARB

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This SAR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-116 amino acids from the Central region of human SAR1B.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-SAR1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene is a small GTPase that
acts as a homodimer. The encoded protein is activated by the
guanine nucleotide exchange factor PREB and is involved in protein
transport from the endoplasmic reticulum to the Golgi. This protein
is part of the COPII coat complex. Defects in this gene are a cause
of chylomicron retention disease (CMRD), also known as Anderson
disease (ANDD). Two transcript variants encoding the same protein
have been found for this gene.

Signal Transduction

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Flow Cytometry (FC/FACS)

IHC~~1:50~100
WB~~1:1000

SAR1B antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SAR1B antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

SAR1B Antibody (Center) western blot analysis in MCF-7,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SAR1B antibody detected the SAR1B protein (arrow).

SAR1B Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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secretion associated, Ras related GTPase 1B

GTP-binding protein SAR1b

GTP-binding protein SAR1b

SARA2; SARB; GTBPB  [Similar Products]

SAR1B_HUMAN

The protein encoded by this gene is a small GTPase that acts as a homodimer. The encoded protein is activated by the guanine nucleotide exchange factor PREB and is involved in protein transport from the endoplasmic reticulum to the Golgi. This protein is part of the COPII coat complex. Defects in this gene are a cause of chylomicron retention disease (CMRD), also known as Anderson disease (ANDD). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

SAR1B: Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD); also known as Anderson disease (ANDD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The condition is characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets. Belongs to the small GTPase superfamily. SAR1 family.

Protein type: G protein, monomeric, SAR1; Motility/polarity/chemotaxis; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: endoplasmic reticulum membrane; cytosol

Molecular Function: GTPase activity; GTP binding; metal ion binding

Biological Process: COPII coating of Golgi vesicle; intracellular protein transport; ER to Golgi vesicle-mediated transport; antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification

Disease: Chylomicron Retention Disease

Cefalu, A.B., et al. Metab. Clin. Exp. 59(4):463-467(2010)
Silvain, M., et al. Clin. Genet. 74(6):546-552(2008)
Charcosset, M., et al. Mol. Genet. Metab. 93(1):74-84(2008)
Jardim, D.L., et al. J. Biochem. Mol. Biol. 38(3):328-333(2005)
Jones, B., et al. Nat. Genet. 34(1):29-31(2003)

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