CD274, Low Endotoxin, Monoclonal Antibody

Anti -CD274, Low Endotoxin (B7-h1 Protein, B7-H1, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 9; NC_000009.11 (5450503..5470567). Location: 9p24

Monoclonal

IgG2a

9E32

Rat

Recognizes mouse CD274

Purified by Protein G Chromatography. Endotoxin: <=0.01EU/ug.

Supplied as a liquid in PBS. No preservative added.

1mg/ml (lot specific)

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-CD274 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naïve T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.

Antibodies; Abs to CD Markers

Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Functional Assays.

Dilution: Flow Cytometry: Neat-1:200. 10ul labels 1x10e6 cells in 100ul.
Immunohistochemistry: Frozen sections
Optimal diluitons to be determined by the researcher.

Flow Cytometry analysis of mouse lymphocytes using MBS607584

14,775 Da[Similar Products]

CD274 molecule

programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; OTTHUMP00000021029; programmed death ligand 1

CD274

Q0GN75_HUMAN

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: integral to membrane; plasma membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction

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