Product Name
HepA-Related Protein (SMARCAL1), Polyclonal Antibody
Full Product Name
HepA-Related Protein (HARP, SMARCAL1, EC 3.6.1.-, SIOD)
Product Synonym Names
Anti -HepA-Related Protein (HARP, SMARCAL1, EC 3.6.1.-, SIOD)
Product Gene Name
anti-SMARCAL1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
chromosome: 2; Location: 2q35
3D Structure
ModBase 3D Structure for Q9NZC9
Specificity
Recognizes Human HepA-related Protein (HARP) (SMARCAL1). Species Crossreactivity: Human.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Immunogen
Synthetic peptide, KLH-conjugated. Epitope: aa 80-96 of human SmarcAL1 protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-SMARCAL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-SMARCAL1 antibody
Antibodies; Abs to Chromatin Proteins
Applications Tested/Suitable for anti-SMARCAL1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-SMARCAL1 antibody
Suitable for use in ELISA, IImmunofluorescence, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry: 5ug/ml
Western Blot: 1:500-1:1000
ELISA: 1:16,000-1:64,000
NCBI/Uniprot data below describe general gene information for SMARCAL1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
Q9NZC9
[Other Products]
UniProt Secondary Accession #
Q53R00; Q96AY1; Q9NXQ5; Q9UFH3; Q9UI93; A6NEH0[Other Products]
UniProt Related Accession #
Q9NZC9[Other Products]
Molecular Weight
105,938 Da[Similar Products]
NCBI Official Full Name
HepA-related protein HARP
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
NCBI Official Symbol
SMARCAL1 [Similar Products]
NCBI Official Synonym Symbols
HARP; HHARP; SMARCAL1
[Similar Products]
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1; OTTHUMP00000206800; HepA-related protein; SMARCA-like protein 1; ATP-driven annealing helicase; sucrose nonfermenting protein 2-like 1
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1
UniProt Synonym Protein Names
HepA-related protein; hHARP; Sucrose nonfermenting protein 2-like 1
UniProt Gene Name
SMARCAL1 [Similar Products]
UniProt Synonym Gene Names
HARP [Similar Products]
UniProt Entry Name
SMAL1_HUMAN
NCBI Summary for SMARCAL1
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency. [provided by RefSeq]
UniProt Comments for SMARCAL1
SMARCAL1: ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD). SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema. Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
Protein type: EC 3.6.1.-; EC 3.6.4.-; Helicase
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: nucleoplasm; DNA replication factor A complex; nucleus
Molecular Function: DNA-dependent ATPase activity; protein binding; DNA binding; helicase activity; ATP binding
Biological Process: regulation of transcription from RNA polymerase II promoter; DNA strand renaturation; chromatin modification; DNA repair; response to DNA damage stimulus; DNA metabolic process; replication fork processing
Disease: Immunoosseous Dysplasia, Schimke Type
Research Articles on SMARCAL1
1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
2. SMARCAL1 is a novel DNA damage-binding protein involved in replication fork stabilization.
3. ARP is recruited to stalled replication forks via its direct interaction with Replication protein A (RPA)
4. the interaction of HARP with RPA increases the concentration of annealing helicase activity in the vicinity of ssDNA regions to facilitate processes such as DNA repair
5. SMARCAL1 directly interacts with Replication protein A (RPA) and is recruited to sites of DNA damage in an RPA-dependent manner
6. SMARCAL1
7. Donor serum SMARCAL1 may serve as a specific, sensitive, and noninvasive predictive marker in the assessment of cardiac graft quality.
8. SMARCAL1 mutations: a cause of prepubertal idiopathic steroid-resistant nephrotic syndrome. (Case Report).
9. SMARCAL1 binds chromatin in vivo and that Schimke immuno-osseous dysplasia arises from impairment of diverse SMARCAL1 functions
10. study found that HARP is an ATP-dependent annealing helicase that rewinds single-stranded DNA bubbles that are stably bound by replication protein A
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