EIF2A, Polyclonal Antibody

Anti -EIF2A, phosphorylated (Ser49) (Eukaryotic Translation Initiation Factor 2A, eIF-2A, 65kD Eukaryotic Translation Initiation Factor 2A, CDA02, MSTP004, MSTP089)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 3; NC_000003.11 (150264574..150303803). Location: 3q25.1

Polyclonal

IgG

Rabbit

Recognizes human eIF2 alpha when phosphorylated at serine 49. Species Cross-Reactivity: mouse, rat.

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-EIF2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

EIF-2alpha, also known as eukaryotic translation initiation factor 2alpha, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF-2alpha. Phosphorylated eIF-2 alpha inhibits cell growth and induces apoptosis. Phosphorylation of eIF-2alpha occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses. EIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36kD), beta (38kD), and gamma (52kD).

Antibodies; Abs to Translation Initiation Factors

Immunohistochemistry (IHC)

Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry:1:50-100

64,990 Da[Similar Products]

eukaryotic translation initiation factor 2A, 65kDa

eukaryotic translation initiation factor 2A; OTTHUMP00000216446; OTTHUMP00000216447; OTTHUMP00000216448; OTTHUMP00000216449; 65 kDa eukaryotic translation initiation factor 2A

Eukaryotic translation initiation factor 2A

EIF2A_HUMAN

EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM]

EIF2A: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. Belongs to the WD repeat EIF2A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation

Chromosomal Location of Human Ortholog: 3q25.1

Cellular Component: extracellular space; cytoplasm; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; translation initiation factor activity; ribosome binding; tRNA binding

Biological Process: regulation of translation; ribosome assembly; positive regulation of signal transduction; translational initiation; SREBP-mediated signaling pathway; protein amino acid phosphorylation

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