Product Name
Semaphorin 3E (SEMA3E), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Semaphorin 3E (SEMA3E)
Product Gene Name
anti-SEMA3E antibody
[Similar Products]
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
Immunogen: Semaphorin 3E (SEMA3E) (MBS2018304)
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
APC-CY7 Conjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2083400)
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
PE Conjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2083401)
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
APC Conjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2083402)
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
Cy3 Conjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2083403)
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
FITC Conjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2083404)
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
HRP Conjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2083405)
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Unconjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2018145)
Biotin Conjugated Antibody: Semaphorin 3E (SEMA3E) (MBS2096901)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- LDAGTYFCQT VEHSIVHTVR KITLEIVEEE RVDEMFSKDY
3D Structure
ModBase 3D Structure for O42237
Species Reactivity
Chicken
Specificity
The antibody is a rabbit polyclonal antibody raised against SEMA3E. It has been selected for its ability to recognize SEMA3E in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant SEMA3E (Leu651~Asp740) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083402
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customerâs specifications, please inquire.
Other Notes
Small volumes of anti-SEMA3E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SEMA3E antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-SEMA3E antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB) of anti-SEMA3E antibody
Western Blot: Sample: Recombinant protein.

NCBI/Uniprot data below describe general gene information for SEMA3E. It may not necessarily be applicable to this product.
NCBI Accession #
NP_989573.1
[Other Products]
NCBI GenBank Nucleotide #
NM_204242.1
[Other Products]
UniProt Primary Accession #
O42237
[Other Products]
UniProt Secondary Accession #
Q90666[Other Products]
UniProt Related Accession #
O42237[Other Products]
Molecular Weight
90,979 Da
NCBI Official Full Name
semaphorin-3E
NCBI Official Synonym Full Names
semaphorin 3E
NCBI Official Symbol
SEMA3E [Similar Products]
NCBI Official Synonym Symbols
semaphorin-3E
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NCBI Protein Information
semaphorin-3E
UniProt Protein Name
Semaphorin-3E
UniProt Synonym Protein Names
Collapsin-5; COLL-5
Protein Family
Semaphorin
UniProt Gene Name
SEMA3E [Similar Products]
UniProt Synonym Gene Names
COLL5; COLL-5 [Similar Products]
UniProt Comments for SEMA3E
Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis. Can down-regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Induces the collapse and paralysis of neuronal growth cones. Plays an important role in ensuring the specificity of synapse formation ().
Research Articles on SEMA3E
1. Results suggest a molecular mechanism whereby ingrowth of retinal axons into the tectum can be regulated by semaphorin 3E/BDNF modulation without disturbing tectal axon growth out of the tectum mediated by Sema3A.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.