A/G-specific adenine DNA glycosylase, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat MUTYH. No significant cross-reactivity or interference between Rat MUTYH and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of MUTYH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283486 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the A/G-specific adenine DNA glycosylase (MUTYH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MUTYH. The ELISA analytical biochemical technique of the MBS283486 kit is based on MUTYH antibody-MUTYH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MUTYH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MUTYH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MUTYH in samples. An antibody specific for MUTYH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUTYH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUTYH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUTYH bound in the initial step. The color development is stopped and the intensity of the color is measured.

57,919 Da

mutY DNA glycosylase

adenine DNA glycosylase

Adenine DNA glycosylase

Myh; rMYH  [Similar Products]

an DNA repair enzyme that excises adenine residues misincorporated opposite the oxidized base [RGD, Feb 2006]

MUTYH: Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2- OH-A DNA glycosylase activities. Belongs to the Nth/MutY family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 3.2.2.-; Hydrolase; Mitochondrial; Tumor suppressor

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: mitochondrion; nucleus

Molecular Function: 4 iron, 4 sulfur cluster binding; DNA binding; DNA N-glycosylase activity; metal ion binding; MutLalpha complex binding; MutLbeta complex binding; MutSalpha complex binding; MutSbeta complex binding; purine-specific mismatch base pair DNA N-glycosylase activity

Biological Process: base-excision repair; response to oxidative stress

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