Product Name
Aurora Kinase (AURKA), Polyclonal Antibody
Full Product Name
Aurora Kinase Antibody
Product Synonym Names
AIK; ARK1; Aurora-A; Aurora-family kinase 1; Aurora-related kinase 1; Aurora/IPL1-related kinase 1; AYK1; Breast-tumor-amplified kinase; BTAK; IAK1; Ipl1- and aurora-related kinase 1; kinase AurA; Serine/threonine kinase 15; Serine/threonine kinase 6; Ser
Product Gene Name
anti-AURKA antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O14965
Species Reactivity
Human, Mouse, Rat
Specificity
Aurora Kinase Antibody detects endogenous levels of total Aurora Kinase protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human Aurora Kinase.
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-AURKA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AURKA antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-AURKA antibody
WB: 1:500~1:1000
ELISA: 1:5000
Western Blot (WB) of anti-AURKA antibody
Western blot analysis of lysates from 293 cells, treated with serum 20% 15', using Aurora Kinase Antibody. The lane on the right is blocked with the synthesized peptide.
NCBI/Uniprot data below describe general gene information for AURKA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001310232.1
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NCBI GenBank Nucleotide #
NM_001323303.1
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UniProt Primary Accession #
O14965
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UniProt Secondary Accession #
O60445; O75873; Q9BQD6; Q9UPG5; E1P5F9[Other Products]
UniProt Related Accession #
O14965[Other Products]
NCBI Official Full Name
aurora kinase A
NCBI Official Synonym Full Names
aurora kinase A
NCBI Official Symbol
AURKA [Similar Products]
NCBI Official Synonym Symbols
AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
[Similar Products]
NCBI Protein Information
aurora kinase A
UniProt Protein Name
Aurora kinase A
UniProt Synonym Protein Names
Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A
Protein Family
Aurora kinase
UniProt Gene Name
AURKA [Similar Products]
UniProt Synonym Gene Names
AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; ARK-1; Aurora-related kinase 1; hARK1 [Similar Products]
NCBI Summary for AURKA
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for AURKA
Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.
Research Articles on AURKA
1. SIX3 is a novel negative transcriptional regulator and acts as a tumor suppressor that directly represses the transcription of AURKA and AURKB in astrocytoma.
Precautions
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Disclaimer
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