RET, Polyclonal Antibody

Anti-RET Antibody (N-Terminus) IHC-plus; RET; Cadherin family member 12; CDHF12; C-ret; Hirschsprung disease 1; HSCR1; Hydroxyaryl-protein kinase; Oncogene ret; Proto-oncogene c-Ret; RET-ELE1; RET51; Ret proto-oncogene; RET transforming sequence; RET43; Men2-ret; MEN2B; MTC1; Human RET

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Ammonium sulfate precipitation

PBS, 0.09% sodium azide

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Small volumes of anti-RET antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The recombination of RET tyrosine kinase with several genes, such as GOLGA5 and the H4 gene, results in a consitutively active oncogenic protein. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma.

Subfamily:-Cadherin">Family: Protein Kinase
Subfamily: Cadherin

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

IHC-P (5 ug/ml), WB (1:1000)

Anti-RET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot of anti-Ret Pab in SKBR3 cell lysate. Ret (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

119,847 Da

ret proto-oncogene

proto-oncogene tyrosine-protein kinase receptor Ret; proto-oncogene c-Ret; receptor tyrosine kinase; RET transforming sequence; cadherin family member 12; hydroxyaryl-protein kinase; RET receptor tyrosine kinase; rearranged during transfection; cadherin-related family member 16; ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)

Proto-oncogene tyrosine-protein kinase receptor Ret

CDHF12; CDHR16; PTC; RET51  [Similar Products]

RET_HUMAN

This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008]

Ret: a proto-oncogenic receptor tyrosine kinase. Receptor for glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin. Part of a multicompetent receptor complex with other membrane-bound ligand-binding GDNF family receptors (aGFRs). Required for development of the kidney and neural crest-derived cell types. Three alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; EC 2.7.10.1; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); Oncoprotein; TK group; Ret family

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; endosome membrane; receptor complex; lipid raft

Molecular Function: protein binding; protein-tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity; calcium ion binding; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; positive regulation of cell size; positive regulation of transcription, DNA-dependent; membrane protein proteolysis; response to pain; regulation of axonogenesis; signal transduction; neuron maturation; protein amino acid phosphorylation; enteric nervous system development; ureteric bud development; embryonic epithelial tube formation; neuron adhesion; neural crest cell migration; caspase activation; response to drug; regulation of cell adhesion; positive regulation of neuron maturation; posterior midgut development; MAPKKK cascade; positive regulation of cell adhesion mediated by integrin; retina development in camera-type eye; homophilic cell adhesion; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

Disease: Multiple Endocrine Neoplasia, Type Iib; Multiple Endocrine Neoplasia, Type Iia; Thyroid Carcinoma, Papillary; Hirschsprung Disease, Susceptibility To, 1; Thyroid Carcinoma, Familial Medullary

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