PNPLA3, Polyclonal Antibody

Acylglycerol O acyltransferase, Adiponutrin, ADPN, C22orf20, Calcium independent phospholipase A2 epsilon, IPLA2-epsilon, Patatin like phospholipase domain containing 3 phospholipase A2, calcium independent, epsilon

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Immobilized antigen affinity chromatography

Affinity Purified Immunoglobulins

Store at -20°C for long term storage.

Small volumes of anti-PNPLA3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Patatin-like Phospholiipase Domain Containing protein 3(PNPLA3) is an enzyme that acts as a triacylglycerol lipase.The PNPLA3 activity mediates traiacylclycerol hydrolysis in adipocytes. (1)A nonsynonymous polymorphism in PNPLA3 that is strongly associated with hepatic fat content and with elevated serum levels of alanine aminotransferase and aspartate amino transferase have been identified (2) PNPLA3 genetic variation may contribute to ancestry-related an inter-individual differences in hepatic fat content and susceptibility to NAFLD(3).

The PNPLA3-selective antibodies were generated against a synthetic peptide taken within amino acid region 150-200 on human Patatin-like phospholipase domain-containing protein 3.The PNPLA3 synthetic peptide was covalently modified to achiee desired antigenic properties and was conjugated to a carrier protein before used as a immunogen to raise antibodies in rabbits. The PNPLA3 antibodies are affinity purified over immobilized immunogenic peptide affinity matrix and stabilized with preservatives for long-term storage. Antigenic blocking peptide (P-PNPLA3) and western blot positive controls (PC-PNPLA3 )are available. Antibodies can be conjugated to fluorophores or secondary enzymes upon request at nominal cost.

Primary Antibodies; Cell Biology; Lipids; PNPLA3 Antibody

ELISA, IP, WB

DOT Blot (DB): 1:20,000
ELISA (EIA): 1:20,000
Immunohistochemistry (IHC): 1:50-1:100
Immunoprecipitation (IP): 1:200
Western Blot (WB): 1:500-1:750

WB of PNPLA3 antibody (MBS544053) with:
A. Rat liver
B. Mouse kidney
Antibody dilution 1:500 in DiluOBufferTM (FGI-1963).
Apparent MW of PNPLA3 in rat liver is 58 kDa.
Apparent MW of PNPLA3 in mouse kidney is 52 kDa.

Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.

patatin like phospholipase domain containing 3

patatin-like phospholipase domain-containing protein 3

Patatin-like phospholipase domain-containing protein 3

ADPN; C22orf20; iPLA2-epsilon  [Similar Products]

PLPL3_HUMAN

The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008]

PNPLA3: Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. Genetic variations in PNPLA3 are a cause of susceptibility to non-alcoholic fatty liver disease type 1 (NAFLD1). A condition characterized by an accumulation of excess triglyceride in the liver, a condition known as hepatic steatosis (or fatty liver), which is associated with adverse metabolic consequences, including insulin resistance and dyslipidemia. In a subset of individuals, hepatic steatosis promotes an inflammatory response in the liver, referred to as steatohepatitis, which can progress to cirrhosis and liver cancer. NAFLD is the most common form of liver disease in Western countries. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Phospholipase; Lipid Metabolism - glycerophospholipid; Secondary Metabolites Metabolism - limonene and pinene degradation; EC 3.1.1.3; Lipid Metabolism - glycerolipid; Amino Acid Metabolism - phenylalanine; Transferase; Amino Acid Metabolism - tyrosine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: endoplasmic reticulum membrane; integral to membrane; membrane

Molecular Function: acylglycerol O-acyltransferase activity; diolein transacylation activity; lipoprotein lipase activity; mono-olein transacylation activity; phospholipase A2 activity; triacylglycerol lipase activity

Biological Process: triacylglycerol biosynthetic process; triacylglycerol catabolic process

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