1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase epsilon-1, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

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Small volumes of PLCE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2603207 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase epsilon-1 (PLCE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLCE1. The ELISA analytical biochemical technique of the MBS2603207 kit is based on PLCE1 antibody-PLCE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLCE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLCE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

255,047 Da

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1

Kiaa1516; Plce; PLC-epsilon-1  [Similar Products]

PLCE1_MOUSE

PLCE1: a widely expressed bifunctional enzyme with phospholipase activity and Ras guanine-exchange factor (RasGEF) activity. Catalyzes the release of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) from 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. Is an effector of heterotrimeric G protein activation. May play a role in cell survival, cell growth, actin organization and T-cell activation. Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs. Interacts with RHOA. Interacts with IQGAP1, HRAS, RAP1A, RAP2A, RAP2B and RRAS. Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Requires calcium for activiation. The Ras-associating domain 1 is degenerate and may not bind HRAS. The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A, RAP2A and RAP2B and recruitment of HRAS to the cell membrane. The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway. Defects in PLCE1 are the cause of nephrotic syndrome type 3 (NPHS3). Nephrotic syndrome, a malfunction of the kidney glomerular filter, leads to proteinuria, hypoalbuminemia, edema. Overexpressed during heart failure. A GWAS study identified an association between esophageal squamous cell carcinoma and a common DNA variant in the PLCE1 gene in East Asians. Two isoforms of the human protein are produced by alternative splicing. Isoform 1 is broadly expressed and only absent in peripheral blood leukocytes. Isoform 2 is specifically expressed in placenta, lung and spleen. Isoform 1 and isoform 2 associate with Golgi membranes.

Protein type: Phospholipase; Carbohydrate Metabolism - inositol phosphate; GAPs; GAPs, Ras; EC 3.1.4.11

Cellular Component: cytoplasm; cytosol; Golgi apparatus; membrane; plasma membrane

Molecular Function: enzyme binding; guanyl-nucleotide exchange factor activity; hydrolase activity; phosphoinositide phospholipase C activity; phospholipase C activity; phosphoric diester hydrolase activity; protein binding; Ras GTPase binding; signal transducer activity

Biological Process: activation of MAPK activity; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); glomerulus development; lipid catabolic process; lipid metabolic process; Ras protein signal transduction; regulation of G-protein coupled receptor protein signaling pathway; regulation of protein kinase activity; regulation of Ras protein signal transduction; signal transduction; small GTPase mediated signal transduction

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