CD40 Ligand, Recombinant Protein

cd40l; cd40 ligand; trap; cd40lg; CD154; TNFSF5

For Research Use Only. Not for use in diagnostic procedures.

E Coli

>98%, as determined by SDS-PAGE and HPLC

Recombinant tPA was lyophilized from a 1 mg/mL 0.2 um filtered solution containing 0.2M L-arginine, 0.1M phosporic acid.

The lyophilized protein is stable for at least 2 years from date of receipt at -20°C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2-8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.

Small volumes of CD40 Ligand recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

CD40 protein is expressed on all B-lymphocytes during various stages of development, activated T- cells and monocytes, follicular dendritic cells, thymic epithelial cells, and various carcinoma cell lines. It is expressed on most mature B-cell malignancies and on some early B-cell acute lymphocytic leukemias. Human CD40 has been found to be the receptor for a protein involved in an X- linked immunodeficiency syndrome. The CD40 ligand is identical with a T-cell restricted cell surface activation antigen designated 5c8. The mouse ligand of CD40 is gp39.Based on its homology with other members of the TNF ligand protein superfamily the protein is referred to also as TNFSF5.

Cytokines

CD40 ligand

CD40L; TNFSF5; TRAP; CD40-L; TRAP  [Similar Products]

CD40L_HUMAN

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; regulation of immune response; leukocyte adhesion; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 1

• the cd40/CD40 Ligand system: linking inflammation with atherothrombosis .J. Am. Coll. Cardiol., Aug 2009; 54: 669 - 677.
• growth inhibition of human multiple myeloma cells by an oncolytic adenovirus carrying the CD40 Ligand transgene .Clin. Cancer Res., Aug 2009; 15: 4847 - 4856.
• matrix metalloproteinase 9 is involved in crohn’s disease-associated platelet hyperactivation through the release of soluble CD40 Ligand .Gut, Jul 2009; 58: 920 - 928.
• distinct roles of cd28- and CD40 Ligand-mediated costimulation in the development of protective immunity and pathology during chlamydia muridarum urogenital infection in mice .Infect. Immun., Jul 2009; 77: 3080 - 3089.
• phase i study of intranodal direct injection of adenovirus encoding recombinant cd40-ligand (ad-isf35) in patients with chronic lymphocytic leukemia .ASCO Meeting Abstracts, May 2009; 27: 3003.