Product Name
CD40L (CD40LG), Monoclonal Antibody
Full Product Name
MAb to CD40L
Product Synonym Names
Monoclonal Antibody to Human CD40 Ligand
Product Gene Name
anti-CD40LG antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: X; NC_000023.10 (135730336..135742549). Location: Xq26
3D Structure
ModBase 3D Structure for P29965
Host
Host: Mouse. Hybridization of Sp 2/0 myeloma cells with spleen cells from BALB/c mice.
Source: Ascites
Specificity
CD40L
Soluble human CD40L. Can be used for detection of human sCD40L in serum.
Purity/Purification
>90% pure. Protein A chromatography
Form/Format
Purified, Liquid
Concentration
2.6mg/ml (
Sigma protein assay kit) (lot specific)
Immunogen
Recombinant human soluble CD40 Ligand/TRAP Analog conjugates with BSA
Affinity Constant
Not determined
Preservative
0.1% Sodium azide
Important Note
Centrifuge before opening to ensure complete recovery of vial contents.
Warnings
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Store at 2 to 8 degree C.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-CD40LG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-CD40LG antibody
Monoclonal Antibodies to Human CDs
Applications Tested/Suitable for anti-CD40LG antibody
EIA/ELISA, Western Blot
NCBI/Uniprot data below describe general gene information for CD40LG. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000065.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000074.2
[Other Products]
UniProt Primary Accession #
P29965
[Other Products]
UniProt Related Accession #
P29965; Q3L8U2; Q3LFI8; Q45QX2[Other Products]
Molecular Weight
29,274 Da[Similar Products]
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG [Similar Products]
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
[Similar Products]
NCBI Protein Information
CD40 ligand; CD40-L; OTTHUMP00000024130; OTTHUMP00000024131; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5
Protein Family
CD40 ligand
UniProt Gene Name
CD40LG [Similar Products]
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP [Similar Products]
UniProt Entry Name
CD40L_HUMAN
NCBI Summary for CD40LG
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq]
UniProt Comments for CD40LG
Function: Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Ref.10Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. Ref.10
Subunit structure: Homotrimer.
Subcellular location: Cell membrane; Single-pass type II membrane protein. CD40 ligand, soluble form: Secreted.
Tissue specificity: Specifically expressed on activated CD4+ T-lymphocytes.
Post-translational modification: The soluble form derives from the membrane form by proteolytic processing.N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.Not O-glycosylated. Ref.9
Involvement in disease: Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [
MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Ref.3 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21
Sequence similarities: Belongs to the tumor necrosis factor family.
Research Articles on CD40LG
1. this study demonstrates high CD154 and FAS expression as a characteristic feature of SLE.
Precautions
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Disclaimer
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