Connexin 31, Polyclonal Antibody

Anti -Connexin 31

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 1; NC_000001.10 (35246790..35251967). Location: 1p34

Polyclonal

IgG

Rabbit

Recognizes the ~32kD mouse connexin 31 on Western Blots. The band observed at ~62kD is probably due to dimerization of connexin 31. Several higher bands of unknown origin are observed in Western Blots of mouse skin homogenates. Reactivity has been confirmed with mouse skin homogenate.

Affinity Purified
Purified by Immunoaffinity chromatgraphy.

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-GJB3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Connexin 31 (Cx31) is a 270 amino acid gap junction protein in mouse and rat with a predicted molecular weight of ~31kD.1 Connexin 31 is encoded by the gene Gjb3, and is expressed in a complex pattern throughout mouse development. During early mouse development, Cx31 is present in both the inner cell mass and the trophectoderm, but it is restricted to cells derived from the trophectoderm lineage after implantation. In ***** female rodents, Cx31 is expressed in the granular layer of the placenta, and the lack of Cx31 leads to transient dysmorphogenesis affecting embryonic survival. In ***** male rodents, Cx31 is expressed in the seminiferous epithelium of testis and may play a role in cell-cell communication during spermatogenensis.

Antibodies; Abs to Connexin, Junction Proteins

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Dilution: Immunofluorescence: 1-3ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry: 1-3ug/ml on fixed and paraformaldehyde-perfued tissues

30,818 Da[Similar Products]

gap junction protein, beta 3, 31kDa

gap junction beta-3 protein; connexin 31; connexin-31; OTTHUMP00000004183; OTTHUMP00000004184

Gap junction beta-3 protein

CX31  [Similar Products]

CXB3_HUMAN

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

GJB3: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJB3 are a cause of erythrokeratodermia variabilis (EKV). EKV is a genodermatosis characterized by the appearance of two independent skin lesions: transient figurate erythematous patches and hyperkeratosis that is usually localized but occasionally occurs in its generalized form. Clinical presentation varies significantly within a family and from one family to another. Palmoplantar keratoderma is present in around 50% of cases. Defects in GJB3 are the cause of deafness autosomal dominant type 2B (DFNA2B). DFNA2 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: connexon complex; cytoplasm; integral to membrane; gap junction

Molecular Function: gap junction channel activity

Biological Process: skin development; sensory perception of sound; in utero embryonic development; cell communication; transmembrane transport; placenta development

Disease: Deafness, Autosomal Recessive 1a; Deafness, Autosomal Dominant 2b; Erythrokeratodermia Variabilis Et Progressiva

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.