Product Name
BOULE/BOLL, Polyclonal Antibody
Full Product Name
Goat anti-BOULE/BOLL Antibody
Product Synonym Names
BOLL; bol, boule-like (Drosophila); BOULE; boule; BOLL antibody; bol; boule-like (Drosophila) antibody; BOULE antibody; boule antibody; BOULE / BOLL
Product Gene Name
anti-BOLL antibody
[Similar Products]
Product Synonym Gene Name
BOULE[Similar Products]
Antibody/Peptide Pairs
BOULE / BOLL peptide (MBS426007) is used for blocking the activity of BOULE/BOLL antibody (MBS421005)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
QPEPIKTVWS IHY
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QPEPIKTVWSIHY, from the C Terminus of the protein sequence according to NP_932074.1; NP_149019.1.
Note
This antibody is expected to recognize both reported isoforms (NP_932074.1; NP_149019.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-BOLL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-BOLL antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-BOLL antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6ug/ml.
Western Blot: Preliminary experiments gave an approx 22kDa band in Human Testis lysates after 1.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.6kDa according to NP_932074.1 and 31.3kDa according to NP_149019.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide. However, literature reports a 36kDa band in Human and Mouse Testes (PMID: 11390979).
Immunohistochemistry (IHC) of anti-BOLL antibody
(4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
NCBI/Uniprot data below describe general gene information for BOLL. It may not necessarily be applicable to this product.
NCBI Accession #
NP_932074.1
[Other Products]
NCBI Related Accession #
Manufactured in an ISO 9001:2008 Certified Laboratory.NP_149019.1[Other Products]
NCBI GenBank Nucleotide #
NM_197970.2
[Other Products]
UniProt Secondary Accession #
Q0JW32; Q53T62; Q969U3; B4DZA4[Other Products]
UniProt Related Accession #
Q8N9W6[Other Products]
Molecular Weight
19,256 Da
NCBI Official Full Name
protein boule-like isoform 1
NCBI Official Synonym Full Names
boule homolog, RNA binding protein
NCBI Official Symbol
BOLL [Similar Products]
NCBI Official Synonym Symbols
BOULE
[Similar Products]
NCBI Protein Information
protein boule-like
UniProt Protein Name
Protein boule-like
Protein Family
Protein boule
UniProt Gene Name
BOLL [Similar Products]
UniProt Synonym Gene Names
BOULE [Similar Products]
UniProt Entry Name
BOLL_HUMAN
NCBI Summary for BOLL
This gene belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for BOLL
BOLL: Probable RNA-binding protein, which may be required during spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Belongs to the RRM DAZ family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Translation
Chromosomal Location of Human Ortholog: 2q33
Cellular Component: cytoplasm
Molecular Function: nucleotide binding; protein binding; RNA binding; translation activator activity
Biological Process: cell differentiation; meiosis; multicellular organismal development; positive regulation of translational initiation; spermatogenesis
Research Articles on BOLL
1. Our study demonstrated an oncogenic role of BOLL in CRC despite tumor-specific promoter hypermethylation.
Precautions
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Disclaimer
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