Product Name
Collagen II (COL2A1), Polyclonal Antibody
Full Product Name
Anti-Collagen II antibody
Product Synonym Names
Collagen alpha-1(II) chain; collagen, type II, alpha 1; Alpha 1 type II collagen antibody; Alpha-1 type II collagen antibody; AOM antibody; Cartilage collagen antibody; Chondrocalcin antibody; CO2A1_HUMAN antibody; COL11A3 antibody; COL2A1 antibody; COL2A1 protein antibody; Collagen alpha 1(II) chain precursor antibody; Collagen II alpha 1 polypeptide antibody; Collagen type II alpha 1(primary osteoarthritis spondyloepiphyseal dysplasia congenital) antibody; MGC131516 antibody; SEDC antibody
Product Gene Name
anti-COL2A1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P28481
Species Reactivity
Mouse, Rat
Purity/Purification
Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen II1222-1241aa AFAGLGQREKGPDPMQYMRA), different from the related rat sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-COL2A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-COL2A1 antibody
Description: Rabbit IgG polyclonal antibody for Collagen alpha-1(II) chain(COL2A1) detection. Tested with WB, IHC-F in Mouse, Rat.
Background: Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
Applications Tested/Suitable for anti-COL2A1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Formalin
Immunohistochemistry (IHC) of anti-COL2A1 antibody
Anti-Collagen II antibody, MBS176768, IHC(F)
IHC(F): Rat Trachea Tissue
Western Blot (WB) of anti-COL2A1 antibody
Anti-Collagen II antibody, MBS176768, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 1: Rat Brain Tissue Lysate
NCBI/Uniprot data below describe general gene information for COL2A1. It may not necessarily be applicable to this product.
NCBI Accession #
P28481.2
[Other Products]
UniProt Primary Accession #
P28481
[Other Products]
UniProt Secondary Accession #
Q61428; Q62031; Q62032; Q62033; Q641K3; Q6LDB1; Q6LDI8; Q6LDI9; Q80VY3; Q80X38; Q8CEF7[Other Products]
UniProt Related Accession #
P28481[Other Products]
Molecular Weight
134,722 Da
NCBI Official Full Name
Collagen alpha-1(II) chain
NCBI Official Synonym Full Names
collagen, type II, alpha 1
NCBI Official Symbol
Col2a1 [Similar Products]
NCBI Official Synonym Symbols
Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; M100856; Rgsc856
[Similar Products]
NCBI Protein Information
collagen alpha-1(II) chain; collagen alpha-1(II) chain; chondrocalcin; alpha-1 type II collagen; disproportionate micromelia; procollagen, type II, alpha 1
UniProt Protein Name
Collagen alpha-1(II) chain
UniProt Synonym Protein Names
Alpha-1 type II collagenCleaved into the following 2 chains:Collagen alpha-1(II) chain; Chondrocalcin
UniProt Gene Name
Col2a1 [Similar Products]
UniProt Entry Name
CO2A1_MOUSE
UniProt Comments for COL2A1
COL2A1: the alpha-1 chain of type II collagen, an extra-cellular matrix protein found in cartilage and the vitreous humor of the eye. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Chondrocalcin is the calcium binding C-propeptide of this collagen molecule. Defects in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. There are two transcripts identified for this gene. Belongs to the fibrillar collagen family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; Extracellular matrix
Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; collagen; collagen type II; cytoplasm; extracellular region; basement membrane
Molecular Function: identical protein binding; extracellular matrix structural constituent; metal ion binding; platelet-derived growth factor binding
Biological Process: heart morphogenesis; proteoglycan metabolic process; inner ear morphogenesis; collagen fibril organization; palate development; limb morphogenesis; sensory perception of sound; visual perception; skeletal morphogenesis; regulation of gene expression; cartilage development; tissue homeostasis; chondrocyte differentiation; inner ear development; skeletal development; cartilage condensation; endochondral ossification
Research Articles on COL2A1
1. Endoplasmic reticulum stress-unfolding protein response-apoptosis cascade causes chondrodysplasia in a col2a1 p.Gly1170Ser mutated mouse model.
Precautions
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Disclaimer
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