Full Product Name
EXT1 siRNA (Mouse)
Product Synonym Names
Exostosin-1; Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; Multiple exostoses protein 1 homolog
Product Gene Name
EXT1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P97464
Specificity
EXT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EXT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of EXT1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EXT1 sirna
siRNA to inhibit EXT1 expression using RNA interference
Applications Tested/Suitable for EXT1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for EXT1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034292.2
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NCBI GenBank Nucleotide #
NM_010162.2
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UniProt Primary Accession #
P97464
[Other Products]
UniProt Secondary Accession #
Q61546[Other Products]
UniProt Related Accession #
P97464[Other Products]
Molecular Weight
86,308 Da
NCBI Official Full Name
exostosin-1
NCBI Official Synonym Full Names
exostoses (multiple) 1
NCBI Official Symbol
Ext1 [Similar Products]
NCBI Official Synonym Symbols
AA409028
[Similar Products]
NCBI Protein Information
exostosin-1
UniProt Protein Name
Exostosin-1
UniProt Synonym Protein Names
Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; Multiple exostoses protein 1 homolog
UniProt Gene Name
Ext1 [Similar Products]
UniProt Entry Name
EXT1_MOUSE
UniProt Comments for EXT1
EXT1: Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Defects in EXT1 are a cause of hereditary multiple exostoses type 1 (EXT1). EXT is a genetically heterogeneous bone disorder caused by genes segregating on human chromosomes 8, 11, and 19 and designated EXT1, EXT2 and EXT3 respectively. EXT is a dominantly inherited skeletal disorder primarily affecting endochondral bone during growth. The disease is characterized by formation of numerous cartilage-capped, benign bone tumors (osteocartilaginous exostoses or osteochondromas) that are often accompanied by skeletal deformities and short stature. In a small percentage of cases exostoses have exhibited malignant transformation resulting in an osteosarcoma or chondrosarcoma. Osteochondromas development can also occur as a sporadic event. Defects in EXT1 are a cause of tricho-rhino-phalangeal syndrome type 2 (TRPS2). A syndrome that combines the clinical features of trichorhinophalangeal syndrome type 1 and multiple exostoses type 1. Affected individuals manifest multiple dysmorphic facial features including large, laterally protruding ears, a bulbous nose, an elongated upper lip, as well as sparse scalp hair, winged scapulae, multiple cartilaginous exostoses, redundant skin, and mental retardation. A chromosomal aberration resulting in the loss of functional copies of TRPS1 and EXT1 has been found in TRPS2 patients. Defects in EXT1 are a cause of chondrosarcoma (CHDSA). It is a malignant neoplasm derived from cartilage cells. Chondrosarcomas range from slow-growing non-metastasizing lesions to highly aggressive metastasizing sarcomas. Belongs to the glycosyltransferase 47 family.
Protein type: EC 2.4.1.225; Motility/polarity/chemotaxis; Tumor suppressor; EC 2.4.1.224; Glycan Metabolism - heparan sulfate biosynthesis; Membrane protein, integral; Transferase
Cellular Component: Golgi apparatus; membrane; endoplasmic reticulum; integral to membrane; integral to endoplasmic reticulum membrane
Molecular Function: transferase activity; acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups; protein homodimerization activity; glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity; glucuronosyltransferase activity; protein heterodimerization activity; N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity; metal ion binding
Biological Process: glycosaminoglycan biosynthetic process; axon guidance; cellular polysaccharide biosynthetic process; ossification; heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; olfactory bulb development; heparan sulfate proteoglycan biosynthetic process; gastrulation; mesoderm development; brain development; endoderm development
Research Articles on EXT1
1. Ext genes and heparan sulfate are needed to establish and maintain perichondrium's phenotype and border function.
Precautions
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Disclaimer
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