Natural resistance-associated macrophage protein 1 (SLC11A1), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of SLC11A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7217788 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Natural resistance-associated macrophage protein 1 (SLC11A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SLC11A1. The ELISA analytical biochemical technique of the MBS7217788 kit is based on SLC11A1 antibody-SLC11A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SLC11A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SLC11A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Signal Transduction

47,292 Da

solute carrier family 11 (proton-coupled divalent metal ion transporter), member 1

natural resistance-associated macrophage protein 1; NRAMP 1; solute carrier family 11 member 1; solute carrier family 11 (sodium/phosphate symporters), member 1; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1

Natural resistance-associated macrophage protein 1

LSH; NRAMP; NRAMP1; NRAMP 1  [Similar Products]

NRAM1_HUMAN

This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

SLC11A1: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. Belongs to the NRAMP family.

Protein type: Membrane protein, integral; Cell surface; Transporter, SLC family; Membrane protein, multi-pass; Vesicle; Transporter

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: phagocytic vesicle membrane; late endosome membrane; lysosome; integral to plasma membrane; late endosome; plasma membrane

Molecular Function: transition metal ion transmembrane transporter activity; protein homodimerization activity; manganese ion transmembrane transporter activity; metal ion:hydrogen antiporter activity

Biological Process: respiratory burst; wound healing; positive regulation of T-helper 1 type immune response; cellular iron ion homeostasis; positive regulation of dendritic cell antigen processing and presentation; manganese ion transport; response to lipopolysaccharide; defense response to Gram-negative bacterium; vacuolar acidification; antigen processing and presentation of peptide antigen; nitrite transport; T cell proliferation during immune response; T cell cytokine production; positive regulation of phagocytosis; interleukin-3 production; inflammatory response; transmembrane transport; positive regulation of cytokine production; interaction with host; mRNA stabilization; L-arginine import; defense response to protozoan; iron ion transport; interleukin-2 production; phagocytosis; macrophage activation; MHC class II biosynthetic process; positive regulation of interferon-gamma production; cellular cadmium ion homeostasis; iron ion homeostasis; response to bacterium; protein import into nucleus, translocation; negative regulation of cytokine production; defense response to bacterium; activation of protein kinase activity; positive regulation of transcription from RNA polymerase II promoter

Disease: Buruli Ulcer, Susceptibility To; Mycobacterium Tuberculosis, Susceptibility To

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