Product Name
NKX2-5, siRNA
Full Product Name
NKX2-5 siRNA (Mouse)
Product Synonym Names
CSX; NKX-2.5; NKX2E; Homeobox protein Nkx-2.5; Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E
Product Gene Name
NKX2-5 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P42582
Specificity
NKX2-5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NKX2-5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of NKX2-5 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
NKX2-5 sirna
siRNA to inhibit NKX2-5 expression using RNA interference
Applications Tested/Suitable for NKX2-5 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for NKX2-5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032726.1
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NCBI GenBank Nucleotide #
NM_008700.2
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UniProt Primary Accession #
P42582
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UniProt Secondary Accession #
P97335[Other Products]
UniProt Related Accession #
P42582[Other Products]
Molecular Weight
34,163 Da
NCBI Official Full Name
homeobox protein Nkx-2.5
NCBI Official Synonym Full Names
NK2 homeobox 5
NCBI Official Symbol
Nkx2-5 [Similar Products]
NCBI Official Synonym Symbols
Csx; Nkx2.5; tinman; Nkx-2.5
[Similar Products]
NCBI Protein Information
homeobox protein Nkx-2.5
UniProt Protein Name
Homeobox protein Nkx-2.5
UniProt Synonym Protein Names
Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E
Protein Family
Homeobox protein
UniProt Gene Name
Nkx2-5 [Similar Products]
UniProt Synonym Gene Names
Csx; Nkx-2.5; Nkx2e [Similar Products]
UniProt Entry Name
NKX25_MOUSE
UniProt Comments for NKX2-5
NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Cellular Component: transcription factor complex; cytoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; histone deacetylase binding; chromatin binding; transcription factor binding; transcription factor activity
Biological Process: heart morphogenesis; transcription from RNA polymerase II promoter; tongue development; ventricular cardiac myofibril development; multicellular organismal development; heart development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; regulation of transcription, DNA-dependent; atrial cardiac muscle cell development; positive regulation of cell proliferation; cardiac muscle morphogensis; thyroid gland development; regulation of cardiac muscle cell proliferation; hemopoiesis; heart looping; cell differentiation; vasculogenesis; cardiac muscle contraction; spleen development; ***** heart development; cardiac muscle development; pharyngeal system development; sarcomere organization; regulation of transcription from RNA polymerase II promoter; heart contraction; positive regulation of cardioblast differentiation; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; positive regulation of heart contraction; endoderm development; negative regulation of apoptosis
Research Articles on NKX2-5
1. role for Nkx2-5 and Gata3 in the ontogeny of the smooth muscle gastric ligaments; gastric ligaments coexpress Gata3, Nkx2-5 and Sox9; germline loss of Gata3 or conditional deletion of Nkx2-5 abrogates Sox9 expression and impairs gastric ligament smooth muscle development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
