Product Name
Nkx2-5, Polyclonal Antibody
Full Product Name
Nkx2-5 antibody - N-terminal region
Product Gene Name
anti-NKX2-5 antibody
[Similar Products]
Product Synonym Gene Name
Csx; Nkx-2.5; Nkx2.5; tinman[Similar Products]
Antibody/Peptide Pairs
Nkx2-5 peptide (MBS3228349) is used for blocking the activity of Nkx2-5 antibody (MBS3203379)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Synthetic peptide located within the following region: MFPSPALTPT PFSVKDILNL EQQQRSLASG DLSARLEATL APASCMLAAF
3D Structure
ModBase 3D Structure for P42582
Species Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Rabbit, Rat
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Homology
Cow: 86%; Dog: 86%; Guinea Pig: 86%; Human: 80%; Mouse: 100%; Rabbit: 86%; Rat: 93%
Immunogen
The immunogen is a synthetic peptide directed towards the n terminal region of mouse Nkx2-5
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-NKX2-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NKX2-5 antibody
This is a rabbit polyclonal antibody against Nkx2-5. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development. It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.
Product Categories/Family for anti-NKX2-5 antibody
Polyclonal; Transcription Factor; Transcription Regulation; Cardiovascular; Developmental Biology; Neuroscience; Disease Related; Transcription Factors; Cell Differentiation;
Applications Tested/Suitable for anti-NKX2-5 antibody
Western Blot (WB)
Western Blot (WB) of anti-NKX2-5 antibody
WB Suggested Anti-Nkx2-5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Mouse Thymus

NCBI/Uniprot data below describe general gene information for NKX2-5. It may not necessarily be applicable to this product.
NCBI Accession #
NP_032726
[Other Products]
NCBI GenBank Nucleotide #
NM_008700
[Other Products]
UniProt Primary Accession #
P42582
[Other Products]
UniProt Related Accession #
P42582[Other Products]
NCBI Official Full Name
homeobox protein Nkx-2.5
NCBI Official Synonym Full Names
NK2 homeobox 5
NCBI Official Symbol
Nkx2-5 [Similar Products]
NCBI Official Synonym Symbols
Csx; Nkx2.5; tinman; Nkx-2.5
[Similar Products]
NCBI Protein Information
homeobox protein Nkx-2.5
UniProt Protein Name
Homeobox protein Nkx-2.5
UniProt Synonym Protein Names
Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E
Protein Family
Homeobox protein
UniProt Gene Name
Nkx2-5 [Similar Products]
UniProt Synonym Gene Names
Csx; Nkx-2.5; Nkx2e [Similar Products]
UniProt Entry Name
NKX25_MOUSE
Research Articles on NKX2-5
1. Study shows that deletion of the gene encoding the transcription factor Nkx2-5 at critical steps during trabecular development recapitulates pathological features of hypertrabeculation, providing the first model of ventricular non-compaction cardiomyopathy in ***** mice.
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Disclaimer
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