Product Name
CD46, Monoclonal Antibody
Popular Item
Full Product Name
CD46 (Membrane Cofactor Protein) Mouse Monoclonal Antibody
Product Synonym Names
AHUS2; CD46 antigen complement regulatory protein; Complement membrane cofactor protein; MCP; Measles virus receptor; Membrane cofactor protein; MIC10; TLX; Trophoblast leucocyte common antigen
Product Gene Name
anti-CD46 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P15529
Specificity
CD46 acts as a cofactor for complement factor I, a serine protease, which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 acts as a co-stimulatory factor for T-cells, which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Cellular Localization
Cell Surface
Immunogen
Recombinant human CD46 protein
Hu-Chromosome Location
1q32.2
Positive Control
HeLa, K-562 or MOLT-4 cells. Kidney
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Other Notes
Small volumes of anti-CD46 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD46 antibody
Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-CD46 antibody
Functional Studies (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Flow Cytometry (FC/FACS of anti-CD46 antibody
FCM staining of human PBMC's using CD46 Monoclonal Antibody (122.2).

NCBI/Uniprot data below describe general gene information for CD46. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002380.3
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NCBI GenBank Nucleotide #
NM_002389.4
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UniProt Primary Accession #
P15529
[Other Products]
UniProt Secondary Accession #
Q15429; Q53GV9; Q5HY94; Q5VWS6; Q5VWS7; Q5VWS8; Q5VWS9; Q5VWT0; A0T1T0; A0T1T1; A0T1T2[Other Products]
UniProt Related Accession #
P15529[Other Products]
Molecular Weight
56-66kDa
NCBI Official Full Name
membrane cofactor protein isoform 1
NCBI Official Synonym Full Names
CD46 molecule
NCBI Official Symbol
CD46 [Similar Products]
NCBI Official Synonym Symbols
MCP; TLX; AHUS2; MIC10; TRA2.10
[Similar Products]
NCBI Protein Information
membrane cofactor protein
UniProt Protein Name
Membrane cofactor protein
UniProt Synonym Protein Names
TLX; Trophoblast leukocyte common antigen; CD_antigen: CD46
Protein Family
Membrane cofactor protein
UniProt Gene Name
CD46 [Similar Products]
UniProt Synonym Gene Names
MCP; MIC10 [Similar Products]
UniProt Entry Name
MCP_HUMAN
NCBI Summary for CD46
The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
UniProt Comments for CD46
CD46: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. 16 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: cell surface; focal adhesion; integral to plasma membrane; plasma membrane
Molecular Function: cadherin binding; protein binding; receptor activity
Biological Process: adaptive immune response; interleukin-10 production; positive regulation of interleukin-10 production; positive regulation of memory T cell differentiation; positive regulation of regulatory T cell differentiation; positive regulation of T cell proliferation; regulation of complement activation; regulation of Notch signaling pathway; T cell mediated immunity
Product References and Citations for anti-CD46 antibody
Iwata, K., et al. 1995. Diversity of sites for measles virus binding and for inactivation of complement C3b and C4b on membrane cofactor protein CD46. J. Biol. Chem. 270: 15148-15152. Liszewski, M.K., et al. 1996. Control of the complement system. Adv. Immunol. 61: 201-283. Liszewski, M.K., et al. 1996. Membrane cofactor protein (MCP, CD46). Isoforms differ in protection against the classical pathway of complement. J. Immunol. 156: 4415-442
Research Articles on CD46
1. CD46 controls both Th1 activation and regulation via a miR-150-dependent mechanism.
Precautions
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Disclaimer
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