Full Product Name
TCN2 siRNA (Human)
Product Synonym Names
TC2; Transcobalamin-2; TC-2; Transcobalamin II; TC II; TCII
Product Gene Name
TCN2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P20062
Specificity
TCN2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TCN2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TCN2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TCN2 sirna
siRNA to inhibit TCN2 expression using RNA interference
Applications Tested/Suitable for TCN2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TCN2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000346.2
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NCBI GenBank Nucleotide #
NM_000355.3
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UniProt Primary Accession #
P20062
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UniProt Secondary Accession #
Q96FD4; Q9BVI8; Q9UCI5; Q9UCI6; Q9UDM0[Other Products]
UniProt Related Accession #
P20062; AAB25526[Other Products]
Molecular Weight
44,421 Da
NCBI Official Full Name
transcobalamin-2 isoform 1
NCBI Official Synonym Full Names
transcobalamin II
NCBI Official Symbol
TCN2 [Similar Products]
NCBI Official Synonym Symbols
II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
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NCBI Protein Information
transcobalamin-2
UniProt Protein Name
Transcobalamin-2
UniProt Synonym Protein Names
Transcobalamin II; TC II; TCII
Protein Family
Transcobalamin
UniProt Gene Name
TCN2 [Similar Products]
UniProt Synonym Gene Names
TC2; TC-2; TC II; TCII [Similar Products]
UniProt Entry Name
TCO2_HUMAN
NCBI Summary for TCN2
This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Comments for TCN2
TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 22q12.2
Cellular Component: lysosomal lumen; extracellular space; extracellular region; endosome
Molecular Function: metal ion binding; cobalamin binding
Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process
Disease: Transcobalamin Ii Deficiency
Research Articles on TCN2
1. G and TCN2 776C>G) and transcobalamin II receptor (TCblR 1104C>T) polymorphisms in Korean patients with idiopathic recurrent spontaneous abortion">Transcobalamin II (TCN2 67A>G and TCN2 776C>G) and transcobalamin II receptor (TCblR 1104C>T) polymorphisms in Korean patients with idiopathic recurrent spontaneous abortion
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
