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Transcobalamin II, Polyclonal Antibody

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产品名称: Transcobalamin II, Polyclonal Antibody
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简单介绍

Transcobalamin II, Polyclonal Antibody


Transcobalamin II, Polyclonal Antibody  的详细介绍
Product Name

Transcobalamin II (TCN2), Polyclonal Antibody

Full Product Name

Transcobalamin II, Macrocytic Anemia (TCN2) Polyclonal Antibody

Product Synonym Names
TC2; Transcobalamin II
Product Gene Name

anti-TCN2 antibody

[Similar Products]
Matching Pairs
Unconjugated Antibody: Transcobalamin II (MBS2032671)
Immunogen: Transcobalamin II (MBS2033227)
Matching Pairs
Unconjugated Antibody: Transcobalamin II (MBS2032671)
APC-CY7 Conjugated Antibody: Transcobalamin II, Macrocytic Anemia (TCN2) (MBS2078637)
Matching Pairs
Unconjugated Antibody: Transcobalamin II (MBS2032671)
PE Conjugated Antibody: Transcobalamin II, Macrocytic Anemia (TCN2) (MBS2078638)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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3D Structure
ModBase 3D Structure for Q9R0D6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Specificity
The antibody is a rabbit polyclonal antibody raised against TCN2. It has been selected for its ability to recognize TCN2 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Rat
Fragment
TCN2 (Glu19~Met427)
Quality Control
Content: The quality control contains recombinant TCN2 (Glu19~Met427) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2078639
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-TCN2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Applications Tested/Suitable for anti-TCN2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-TCN2 antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-TCN2 antibody
Western Blot: Sample: Rat Serum; Primary Ab: 2ug/ml Rabbit Anti-Rat TCN2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-TCN2 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-TCN2 antibody
Western Blot: Sample: Recombinant protein.
anti-TCN2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-TCN2 antibody
DAB staining on fromalin fixed paraffin- embedded Kidney tissue)
anti-TCN2 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for TCN2. It may not necessarily be applicable to this product.
NCBI GI #
17865614
NCBI GeneID
64365
NCBI Accession #
Q9R0D6.1 [Other Products]
UniProt Primary Accession #
Q9R0D6 [Other Products]
UniProt Related Accession #
Q9R0D6[Other Products]
Molecular Weight
47,420 Da
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NCBI Official Full Name
Transcobalamin-2
NCBI Official Synonym Full Names
transcobalamin 2
NCBI Official Symbol
Tcn2  [Similar Products]
NCBI Official Synonym Symbols
Tc2; Tcn2p
  [Similar Products]
NCBI Protein Information
transcobalamin-2
UniProt Protein Name
Transcobalamin-2
UniProt Synonym Protein Names
Transcobalamin II; TC II; TCII
Protein Family
Transcobalamin
UniProt Gene Name
Tcn2  [Similar Products]
UniProt Synonym Gene Names
TC-2; TC II; TCII  [Similar Products]
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NCBI Summary for TCN2
component of plasma cobalamin (Cbl; vitamin B12) transport to all cells [RGD, Feb 2006]
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UniProt Comments for TCN2
TCN2: Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells. Defects in TCN2 are the cause of transcobalamin II deficiency (TCN2 deficiency). This results in various forms of anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q21

Cellular Component: extracellular space

Molecular Function: cobalamin binding; metal ion binding

Biological Process: cobalamin transport; cobalt ion transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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