Product Name
Filamin A (FLNA), Monoclonal Antibody
Full Product Name
Filamin A antibody
Product Synonym Names
Monoclonal Filamin A; Anti-Filamin A
Product Gene Name
anti-FLNA antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P21333
Species Reactivity
Bovine
Form/Format
Ascites fluid.
Biological Significance
Filamin is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin ath the other. It is present in smooth muscle, fibroblasts, platelets and lymphocytes. Filamin A, encoded by the FLNA gene, is a widely expressed protein that regulates reorganization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes, and second messengers.
Immunogen
Filamin A antibody was raised in mouse using human platelet protein as the immunogen.
Preparation and Storage
Aliquot and store at -20 degree C. Avoid repeated Freeze/Thaw cycles
Other Notes
Small volumes of anti-FLNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-FLNA antibody
Mouse monoclonal Filamin A antibody
Product Categories/Family for anti-FLNA antibody
Cancer
Applications Tested/Suitable for anti-FLNA antibody
Immunoprecipitation (IP), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-FLNA antibody
WB: 1:250-1:1,000
IF: 1:50-1:200
Immunohistochemistry (IHC) of anti-FLNA antibody
Mouse anti-Filamin antibody staining of MeOH fixed fibroblast.
NCBI/Uniprot data below describe general gene information for FLNA. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001447.2
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NCBI GenBank Nucleotide #
NM_001456.3
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UniProt Primary Accession #
P21333
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UniProt Secondary Accession #
Q5HY53; Q5HY55; Q8NF52; E9KL45[Other Products]
UniProt Related Accession #
P21333[Other Products]
Molecular Weight
280,018 Da[Similar Products]
NCBI Official Full Name
filamin-A isoform 1
NCBI Official Synonym Full Names
filamin A, alpha
NCBI Official Symbol
FLNA [Similar Products]
NCBI Official Synonym Symbols
FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
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NCBI Protein Information
filamin-A
UniProt Protein Name
Filamin-A
UniProt Synonym Protein Names
Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Non-muscle filamin
UniProt Gene Name
FLNA [Similar Products]
UniProt Synonym Gene Names
FLN; FLN1; FLN-A; ABP-280 [Similar Products]
UniProt Entry Name
FLNA_HUMAN
NCBI Summary for FLNA
The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]
UniProt Comments for FLNA
FLNA: a ubiquitous cytoskeletal protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Plays an essential role in embryonic cell migration. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactions with filamin A may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking.
Protein type: Actin-binding; Nuclear receptor co-regulator; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: cortical cytoskeleton; focal adhesion; extracellular region; actin filament; trans-Golgi network; cytosol; actin cytoskeleton; membrane; cell soma; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus
Molecular Function: Rho GTPase binding; protein homodimerization activity; Rac GTPase binding; Ral GTPase binding; transcription factor binding; small GTPase binding; actin filament binding; protein binding; signal transducer activity; protein kinase C binding; mu-type opioid receptor binding; glycoprotein binding; SMAD binding
Biological Process: actin crosslink formation; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; negative regulation of transcription factor activity; receptor clustering; mRNA transcription from RNA polymerase II promoter; dopamine receptor, adenylate cyclase inhibiting pathway; establishment of protein localization; platelet degranulation; early endosome to late endosome transport; actin cytoskeleton reorganization; cytoplasmic sequestering of protein; positive regulation of transcription factor import into nucleus; cilium biogenesis; epithelial to mesenchymal transition; negative regulation of protein catabolic process; blood coagulation
Disease: Fg Syndrome 2; Terminal Osseous Dysplasia; Frontometaphyseal Dysplasia; Cardiac Valvular Dysplasia, X-linked; Melnick-needles Syndrome; Heterotopia, Periventricular, Ehlers-danlos Variant; Intestinal Pseudoobstruction, Neuronal, Chronic Idiopathic, X-linked; Heterotopia, Periventricular, X-linked Dominant; Otopalatodigital Syndrome, Type Ii; Otopalatodigital Syndrome, Type I
Research Articles on FLNA
1. The androgen-triggered AR/filamin A complex controls, through Rac 1, the decision of cells to halt cell cycle and migration.
Precautions
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