Glucose-6-Phosphatase, Catalytic, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

No significant cross-reactivity or interference between Human G6PC and analogues was observed.

Store all reagents at 2-8 degree C

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of G6PC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS070740 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucose-6-Phosphatase, Catalytic (G6PC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing G6PC. The ELISA analytical biochemical technique of the MBS070740 kit is based on G6PC antibody-G6PC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect G6PC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, G6PC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated G6PC concentrations in Human serum, plasma and other body fluids. Using Purified Human G6PC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add G6PC and G6PC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of G6PC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

40,484 Da

glucose-6-phosphatase, catalytic subunit

glucose-6-phosphatase; G6Pase; G-6-Pase; G6Pase-alpha; glucose-6-phosphatase alpha

Glucose-6-phosphatase

G6PT; G-6-Pase; G6Pase  [Similar Products]

G6PC_HUMAN

Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]

G6PC: Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels. Defects in G6PC are the cause of glycogen storage disease type 1A (GSD1A). A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia. Belongs to the glucose-6-phosphatase family.

Protein type: Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - starch and sucrose; Phosphatase (non-protein); Membrane protein, integral; Transporter; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 3.1.3.9; Transporter, SLC family; Membrane protein, multi-pass; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: endoplasmic reticulum membrane; integral to membrane; integral to endoplasmic reticulum membrane

Molecular Function: glucose-6-phosphatase activity; phosphotransferase activity, alcohol group as acceptor; phosphate binding

Biological Process: steroid metabolic process; glycogen metabolic process; response to food; phosphorylated carbohydrate dephosphorylation; glycogen catabolic process; multicellular organism growth; glucose 6-phosphate metabolic process; pathogenesis; glucose transport; glucose homeostasis; gluconeogenesis; cholesterol homeostasis; triacylglycerol metabolic process; urate metabolic process; regulation of gene expression; glucose-6-phosphate transport; hexose transport; carbohydrate metabolic process; transmembrane transport

Disease: Glycogen Storage Disease Ia

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